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Determination of Blood Plasma Fluorescence Extinction Coefficients for Dyes used in Three-Compartment Binding Model

机译:三室结合模型中染料的血浆荧光消光系数的测定

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A three-compartment kinetic model for the binding of a ligand to its receptor in tumor tissue has been explained and the kinetic rates of the model are currently being investigated. In order to determine the plasma excretion rates of the dyes of interest, the fluorescence extinction coefficients must be determined. The fluorescence extinction coefficients of the IRDye700DX-carboxylate (IRDye700DX-C) and IRDye800CW-conjugated to EGFR (IRDye800CW-EGF) have been to be 7.98 ×l0~6 μM~(-1) cm~(-1) and 4.73×10~6 μM~(-1) cm~(-1) , respectively. We determined that the linear range of these dyes in the blood plasma of a mouse was 0 - 0.26 μM. Administration of 1 nmol of each of these dyes to a mouse weighing 25-30g (0.04 μM - 0.033 μM, respectively) will result in blood plasma fluorescence in the linear and readable range.
机译:已经解释了在肿瘤组织中用于配体与其受体结合的三室动力学模型,并且目前正在研究该模型的动力学速率。为了确定目标染料的血浆排泄速率,必须确定荧光消光系数。 IRDye700DX-羧酸盐(IRDye700DX-C)和IRDye800CW与EGFR(IRDye800CW-EGF)共轭的荧光消光系数分别为7.98×10〜6μM〜(-1)cm〜(-1)和4.73×10分别为〜6μM〜(-1)cm〜(-1)。我们确定这些染料在小鼠血浆中的线性范围是0-0.26μM。对体重为25-30g(分别为0.04μM-0.033μM)的小鼠施用1 nmol的每种染料将导致血浆荧光处于线性和可读范围内。

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