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首页> 外文会议>Optical methods for tumor treatment and detection: mechanisms and techniques in photodynamic therapy XX >Preparation, characterization and cellular studies of photosensitizer-loaded lipid nanoparticles for photodynamic therapy
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Preparation, characterization and cellular studies of photosensitizer-loaded lipid nanoparticles for photodynamic therapy

机译：载有光敏剂的光敏脂质脂质纳米粒的制备，表征和细胞研究

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PhotoDynamic Therapy (PDT) has been established as a potent and less invasive treatment for different kinds of cancer. Among various attempts to enhance the therapeutics efficacy of PDT, the specific delivery of the PhotoSensitizer (PS) in the tumor is expected to increase its clinical applications, since unwanted accumulation, especially in the skin, impairs the patients' quality of life (prolonged cutaneous photosensitivity). The aim of this study was to engineer Lipid Nanoparticles (LNP) with different sizes and various PS contents, using simple, solvent-free and easily scale up manufacturing processes. Meso-tetra (hydroxyphenyl) chlorin (wTHPC) is one of the most potent photoactive compounds for clinical use and it has been successfully applied in the treatment of various indications, such as the head and neck, prostate and pancreatic cancers. Here, a derivative of wTHPC was efficiently incorporated into the lipid core of LNP, leading to a large range of stable and reproducible wTHPC-loaded LNP with narrow size distribution. The photophysical and photochemical properties of wTHPC-loaded LNP were studied by measuring absorbance and fluorescence spectra, colloidal stability, particle size and zeta potential, as well as singlet oxygen luminescence. The photocytotoxicity of three selected mTHPC-loaded LNP (25 nm, 45 nm and 95 nm of diameter, respectively) was evaluated on MCF-7 cells, in comparison to free wTHPC, under irradiation at 652 nm with a range of light fluence from 1 to 5 J/cm2. All the physico-chemical, photophysical and biological measurements allow us to conclude that LNP is a promising nano-drug delivery system for PDT.
机译：光动力疗法（PDT）已被确立为一种针对各种癌症的有效且微创的疗法。在提高PDT的治疗功效的各种尝试中，光敏剂（PS）在肿瘤中的特异性递送有望增加其临床应用，因为有害的堆积，特别是在皮肤中的堆积会损害患者的生活质量（长时间的皮肤光敏性）。这项研究的目的是使用简单，无溶剂且易于按比例扩大的制造工艺来设计具有不同尺寸和不同PS含量的脂质纳米颗粒（LNP）。中四（羟苯基）二氢卟酚（wTHPC）是临床上最有效的光敏化合物之一，已成功应用于各种适应症的治疗，例如头颈部，前列腺癌和胰腺癌。在这里，wTHPC的衍生物被有效地整合到LNP的脂质核心中，从而产生了范围广泛，稳定且可重现的装载wTHPC的LNP，且粒径分布较窄。通过测量吸光度和荧光光谱，胶体稳定性，粒径和Zeta电位以及单重态氧发光，研究了装载wTHPC的LNP的光物理和光化学性质。与游离的wTHPC相比，在652 nm的辐照下（从1到1）的光通量范围，对三种​​选择的mTHPC加载的LNP（分别为直径25 nm，45 nm和95 nm）的光细胞毒性进行了评估。至5 J / cm2。所有的物理化学，光物理和生物学测量结果都可以使我们得出结论，LNP是用于PDT的有前途的纳米药物递送系统。

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