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首页> 外文会议>Optical methods for tumor treatment and detection: mechanisms and techniques in photodynamic therapy XXI >Photoacoustic imaging of intravenously injected photosensitizer in rat burn models for efficient antibacterial photodynamic therapy
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Photoacoustic imaging of intravenously injected photosensitizer in rat burn models for efficient antibacterial photodynamic therapy

机译：静脉注射光敏剂在大鼠烧伤模型中的光声成像，用于有效的抗菌光动力治疗

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摘要

For efficient photodynamic treatment of wound infection, a photosensitizer must be distributed in the whole infected tissue region. To ensure this, depth profiling of a photosensitizer is necessary in vivo. In this study, we applied photoacoustic (PA) imaging to visualize the depth profile of an intravenously injected photosensitizer in rat burn models, In burned tissue, pharmacokinetics is complicated; vascular occlusion takes place in the injured tissue, while vascular permeability increases due to thermal invasion, In this study, we first used Evans Blue (EB) as a test drug to examine the feasibility of photosensitizer dosimetry based on PA imaging. On the basis of the results, an actual photosensitizer, talaporfin sodium was used. An EB solution was intravenously injected into a rat deep dermal burn model. PA imaging was performed on the wound with 532 nm and 610 nm nanosecond light pulses for visualizing vasculatures (blood) and EB, respectively. Two hours after injection, the distribution of EB-originated signal spatially coincided well with that of blood-originated signal measured after injury, indicating that EB molecules leaked out from the blood vessels due to increased permeability. Afterwards, the distribution of EB signal was broadened in the depth direction due to diffusion. At 12 hours after injection, clear EB signals were observed even in the zone of stasis, demonstrating that the leaked EB molecules were delivered to the injured tissue layer. The level and time course of talaporfin sodium-originated signals were different compared with those of EB-originated signals, showing animal-dependent and/or drug-dependent permeabilization and diffusion in the tissue. Thus, photosensitizer dosimetry should be needed before every treatment to achieve desirable outcome of photodynamic treatment, for which PA imaging can be concluded to be valid and useful.
机译：为了有效地对伤口感染进行光动力治疗，必须在整个感染组织区域内分配光敏剂。为了确保这一点，在体内必须对光敏剂进行深度分析。在这项研究中，我们应用光声（PA）成像技术在大鼠烧伤模型中可视化了静脉注射的光敏剂的深度分布。在烧伤的组织中，药代动力学很复杂。血管阻塞发生在受伤的组织中，而血管渗透性由于热侵袭而增加。在这项研究中，我们首先使用伊文思蓝（EB）作为测试药物来检验基于PA成像的光敏剂剂量测定的可行性。根据结果​​，使用了实际的光敏剂他拉泊芬钠。将EB溶液静脉注射到大鼠深层皮肤烧伤模型中。用532 nm和610 nm纳秒的光脉冲在伤口上进行PA成像，以分别观察脉管系统（血液）和EB。注射后两个小时，EB起源信号的分布在空间上与受伤后测得的血液起源信号的分布在空间上重合，这表明EB分子由于通透性增加而从血管渗出。之后，由于扩散，EB信号的分布在深度方向上变宽。注射后12小时，即使在淤滞区域也观察到了清晰的EB信号，表明泄漏的EB分子已被递送至受伤的组织层。塔拉泊芬钠源信号的水平和时间过程与EB源信号的水平和时间过程不同，表明动物依赖性和/或药物依赖性透化和扩散在组织中。因此，在每次治疗前都需要进行光敏剂剂量测定，以达到理想的光动力治疗效果，可以得出结论，PA成像是有效和有用的。

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