• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques >Intraoperative metastases detection by laser-induced fluorescence spectroscopy
【24h】

Intraoperative metastases detection by laser-induced fluorescence spectroscopy

机译：激光诱导荧光光谱术中术中转移的检测

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: The authors studied the ability of Laser Induced Fluorescence Spectroscopy (LIFS) for the intraoperative identification of metastases using a photosensitizing agent Photofrin II$+r$/ to enhance spectroscopic detection. A He-Cd laser source (442 nm) was used to produce low-power illumination of tissue via a hand-held 400 $mu@m fiberoptic probe. Through the same fiber, reflected and emitted light was returned to an optical multi-channel analyzer (OMA III) for analysis. Spectroscopic signals were displayed on a screen for immediate examination. Lobund Wistar rats, inoculated with Pollard rat adenocarcinoma cells, were used as an animal model. Photofrin II$+r$/ was administered intraperitoneal 24 or 48 hours prior to surgical exploration in doses varying from 0.75-7.5 mg/kg. Metastases detection was performed during abdominal exploration directed to ipsilateral and contralateral inguinal, iliac, para-aortic and renal lymph nodes. Nineteen tissue samples, identified as abnormal by LIFS, were removed for histologic analysis; 11 of these samples were larger than 5mm and histologic examination revealed malignancy in all cases. While LIFS signals showed malignancy in 8 tissue samples with dimensions less than 5mm, histology confirmed this in only 3. However, serial histologic sections were not performed. From the initial results, it was concluded that LIFS detection of malignant tissue is feasible and enhanced by the addition of Photofrin II$+r$/. LIFS may be a promising technique for the intraoperative detection of primary malignant and metastatic tissue.!
机译：摘要：作者研究了使用光敏剂Photofrin II $ + r $ /来增强光谱检测的激光诱导荧光光谱（LIFS）术中鉴定转移灶的能力。 He-Cd激光源（442 nm）用于通过手持式400μm光纤探头产生低功率的组织照明。通过同一根光纤，反射和发射的光返回到光学多通道分析仪（OMA III）中进行分析。光谱信号显示在屏幕上，以便立即检查。将接种了Pollard大鼠腺癌细胞的Lobund Wistar大鼠用作动物模型。在手术前24或48小时内，腹膜内注射Photofrin II $ + r $ /，剂量范围为0.75-7.5 mg / kg。在腹部探查过程中，针对同侧和对侧腹股沟，，主动脉旁和肾淋巴结进行了转移检测。取出19份经LIFS鉴定为异常的组织样品，进行组织学分析。这些样品中有11个大于5mm，所有患者的组织学检查均显示为恶性。尽管LIFS信号在8个尺寸小于5mm的组织样本中显示出恶性肿瘤，但组织学仅在3个组织中证实了这一点。但是，未进行连续的组织学切片。从最初的结果可以得出结论，通过添加Photofrin II $ + r $ /，LIFS检测恶性组织是可行的并且得到了增强。 LIFS可能是术中检测原发性恶性和转移性组织的有前途的技术。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号