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首页> 外文会议>Optical sensing and detection IV >Detection of bacteria using bacteriophage with hollow gold nanostructures immobilized fiber optic sensor
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Detection of bacteria using bacteriophage with hollow gold nanostructures immobilized fiber optic sensor

机译：使用带有中空金纳米结构固定光纤传感器的噬菌体检测细菌

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摘要

Hollow gold nanostructures (HGNS) have been used in variety of optical biosensors due to their inherent advantage of operating at near infra red (NIR) wavelength, large extinction coefficient and high dielectric sensitivity. The absorption wavelength of these nanostructures can be modulated by changing the ratio of hollow region to the core shell thickness. The aim of the present study is to incorporate the properties of HGNS, to develop LSPR based U-bent fiber optic sensor for detection of pathogens. The detection was carried out using an experimental set up consisting of a white light source, 200 μm diameter optical fiber having bend diameter of 1.6 mm ± 0. 2 mm and a spectrometer. The HGNS were immobilized on the decladded portion of the fiber optic probe by chemisorptions. The effective plasmon penetration depth of the HGNS immobilized fiber optic sensor was approximated by using alternating layers of positively and negatively charged polyelectrolytes. The HGNS immobilized U-bent fiber optic sensor was used for detection of E.coli B40 strain using bacteriophage T4. The preliminary experiments were carried out with 10~4 cfu/ml of E.coli B40 and the change in absorbance obtained was approx. 0.042 ± 0.0045 abs. units (n = 3). The response of this sensor was found to be better than spherical gold nanoparticle immobilized sensing platforms.
机译：由于中空金纳米结构（HGNS）具有在近红外（NIR）波长下工作，大消光系数和高介电灵敏度的固有优势，因此已用于各种光学生物传感器。这些纳米结构的吸收波长可以通过改变中空区域与核壳厚度的比例来调节。本研究的目的是结合HGNS的特性，以开发基于LSPR的U型弯曲光纤传感器来检测病原体。使用包括白光源，弯曲直径为1.6 mm±0.2 mm的200μm直径的光纤和光谱仪组成的实验装置进行检测。通过化学吸附将HGNS固定在光纤探针的倾斜部分上。 HGNS固定式光纤传感器的有效等离激元穿透深度通过使用带正电和带负电的聚电解质交替层来估算。 HGNS固定的U型弯曲光纤传感器用于使用噬菌体T4检测大肠杆菌B40菌株。初步实验是用10〜4 cfu / ml的大肠杆菌B40进行的，吸光度的变化约为。 0.042±0.0045绝对单位（n = 3）。发现该传感器的响应优于球形金纳米颗粒固定的传感平台。

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