Optical principle of Ph measurement for detection of auxin flow through cellular membrane

机译：Ph测量的光学原理，用于检测生长素流过细胞膜的情况

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The paper shows an approach to the determination of pH changes of solutions with a fine spatial resolution by means of fiber-optic tapers and fluorescence detection. This approach can be adopted for the determination of auxin flow through celluar membranes. Spectral absorption and fluorescence of pH transducers, namely of fluorescein, carboxyfluorescein, 6,8-dihydroxy-l,3-pyrenedisulfonic acid disodium salt and 2',7'-bis(2-carbonylethyl)-5(6)-carboxyfluorescein, were tested. The approach, based on the determination of a shift of the maxima of their fluorescence peaks, was employed for processing the measured fluorescence data in bulk solutions. Suitable tapered fiber probes were prepared and in vitro demonstrated for pH monitoring in a pH range from 6 to 7.

机译：本文介绍了一种通过光纤锥度和荧光检测来确定具有良好空间分辨率的溶液pH值变化的方法。该方法可用于测定通过细胞膜的植物生长素流量。 pH换能器，即荧光素，羧基荧光素，6,8-二羟基-1,3-吡啶二磺酸二钠盐和2'，7'-双（2-羰基乙基）-5（6）-羧基荧光素的光谱吸收和荧光为经过测试。该方法基于确定其荧光峰的最大值的偏移，用于处理本体溶液中测得的荧光数据。制备了合适的锥形纤维探针，并在体外证明可在6至7的pH范围内进行pH监测。

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来源
《Optical Sensing Technology and Applications; Proceedings of SPIE-The International Society for Optical Engineering; vol.6585》|2007年|65850Y.1-65850Y.7|共7页
会议地点 Prague(CZ)
作者
Ondrej Podrazky; Jan Mrazek; Miroslav Seidl; Ivan Kasik; Petr Tobiska; Vlastimil Matejec; Tomas Martan; Jan Aubrecht;
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作者单位

Institute of Photonics and Electronics ASCR v.v.i., Chaberska 57, Prague 8, Czech Republic, 182 51;

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会议组织
原文格式 PDF
正文语种 eng
中图分类 TP212;
关键词
fluorescence; transducer; immobilization; pH; taper; auxins;

机译：荧光传感器;固定pH值锥度生长素;

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Optical principle of Ph measurement for detection of auxin flow through cellular membrane

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