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首页> 外文会议>Optical technologies in biophysics and medicine XV; and Laser physics and photonics XV >Assessment of neuroglial relationships under photodynamic treatment using fluorescent visualization of giant axons in crayfish ventral nerve cord
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Assessment of neuroglial relationships under photodynamic treatment using fluorescent visualization of giant axons in crayfish ventral nerve cord

机译：荧光可视化小龙虾腹神经索中巨大轴突的光动力治疗下神经胶质关系的评估

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There are lots of publications last time about important role of neuroglial interactions in normal physiology of nervous system. Some of them infer that neuroglial interactions may support survival of neuronal and glial cells. Study the mechanisms of neuroglial interactions needs convenient models which are simple nervous system. They could be easily isolated and studied while retain natural neuroglial relations. The purpose of present work was to estimate glia protective properties of giant axons in simple model - crayfish ventral nerve cord, upon photooxidative stress. To better visualize the axons shape and reveal the periaxonal glial cells, we were filling of the axons with fluorescent label by means of specially devised microinjector. It enabled us to reveal the glial cells which are in close proximity to the axons. Such glial cells was found to have increased survival in compare to more distant ones from the axons under oxidative stress. The obtained data indicate that the crayfish giant axons have glia protective properties, which molecular mechanisms have to be studied.
机译：上次有很多出版物涉及神经胶质相互作用在神经系统正常生理中的重要作用。他们中的一些人推断神经胶质相互作用可能支持神经元和神经胶质细胞的存活。研究神经胶质相互作用的机制需要简单的模型，即简单的神经系统。它们可以很容易地分离和研究，同时保留自然的神经胶质关系。本研究的目的是评估在简单模型-小龙虾腹神经索中受到光氧化应激时胶质细胞的神经胶质保护特性。为了更好地显示轴突的形状并揭示轴突周围的胶质细胞，我们通过专门设计的微型注射器为轴突填充了荧光标记。它使我们能够揭示紧邻轴突的神经胶质细胞。与在氧化应激条件下距离轴突更远的胶质细胞相比，发现这种胶质细胞具有提高的存活率。获得的数据表明，小龙虾的巨大轴突具有胶质细胞的保护特性，其分子机制有待研究。

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