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Trapping and tracking in the presence of phase disturbances

机译：存在相位干扰时的陷波和跟踪

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Functionalized surfaces can affect (bio-) chemical reactions and control spatially the affinity for various binding partners. By either distributing specific binding points, by using biological reconstituted systems on the surface or by investigating whole cells, a surface phase relief is introduced. In the proximity of a trapped particle, these surface scatterers will both disturb the optical trap and the position tracking signal by changing the wave front of the trapping laser. We investigate the influence of an additional scatterer on trapping force, detection sensitivity and local viscous drag by scanning an optically trapped bead (probe) across a structured surface. Using a photonic force microscope, the probe's fluctuation traces are recorded interferometrically in three dimensions with nm precision and at scan rates of several hundred kilohertz with a quadrant photodiode. The phase disturbance located underneath the optical trap alters the interferometric probe position signals and can lead to incorrect interaction measurements. We propose and test a procedure to correct for the phase disturbance of the surface structure. In a roll over experiment, where one nano-sphere rolls over another, we prove the applicability of our phase correction approach. In addition we investigate the influence of small gold dots on the coverslip on trapping parameters which are relevant for specific interaction measurements in biotechnology.
机译：功能化的表面可以影响（生物）化学反应，并在空间上控制对各种结合配偶体的亲和力。通过分布特异性结合点，通过在表面上使用生物重建系统或研究整个细胞，可以引入表面相缓解。在捕获的粒子附近，这些表面散射体将通过更改捕获激光器的波前而干扰光学陷阱和位置跟踪信号。我们通过在结构化表面上扫描光学捕获的磁珠（探针）来研究附加散射体对捕获力，检测灵敏度和局部粘性阻力的影响。使用光子力显微镜，以纳米精度，三维象限光电二极管在几百千赫兹的扫描速率下，以干涉法在三维上记录探头的波动轨迹。位于光阱下方的相位干扰会改变干涉式探头的位置信号，并可能导致不正确的相互作用测量。我们提出并测试一种程序来校正表面结构的相位干扰。在一个翻转实验中，一个纳米球在另一个纳米球上滚动，我们证明了相位校正方法的适用性。此外，我们研究了盖玻片上的小金点对捕获参数的影响，该参数与生物技术中特定的相互作用测量有关。

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