• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Optical Trapping and Optical Micromanipulation II >Analysis of the swimming activity of Pseudomonas aeruginosa by using photonic force microscope
【24h】

Analysis of the swimming activity of Pseudomonas aeruginosa by using photonic force microscope

机译：用光子力显微镜分析铜绿假单胞菌的游泳活动

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Swimming activity of flagella is a main factor of the motility of bacteria. Flagella expressed on the surface of bacterial species serve as a primary means of motility including swimming. We propose to use optical tweezers to analyze the swimming activity of bacteria. The sample bacteria in this work are Pseudomonas aeruginosa, and it is a gram-negative bacterium and it often causes leading to burn wound infections, urinary-tract infections, and pneumonia. The single polar flagellum of P. aeruginosa has been demonstrated to be important virulence and colonization factor of this opportunistic pathogen. We demonstrate a gene to regulate the bacterial swimming activity in P. aeruginosa PAO1 by biological method. However, the change of flagellar morphology was not observed by electron microscopy analysis, suggesting that the gene regulates the flagellar rotation that could not be detected by biological method. Photonic Force Microscope (PFM) first developed by E. Stelzer of European Molecular Biology Laboratory, is an advanced version of optical tweezers Equipped with an optional high-sensitivity quadrant photo detector (QPD), PFM is capable of trapping a micro object and monitoring the prompt and random Brownian fluctuations of the trapped object simultaneously. It exhibits a spatial resolution of a few nanometers to detect the relative position of the probe at an acquisition rate over 1 MHz. By capturing a flagella, we expect the rotation of the flagella could be detected. It is expected that the study of the swimming activity of P. aeruginosa provide potent method for the pathogenic role of the flagella in P. aeruginosa.
机译：鞭毛的游泳活动是细菌运动的主要因素。在细菌物种表面表达的鞭毛是运动的主要手段，包括游泳。我们建议使用光镊来分析细菌的游泳活动。这项工作中的样本细菌是铜绿假单胞菌，它是革兰氏阴性细菌，它经常导致烧伤创面感染，尿路感染和肺炎。铜绿假单胞菌的单极鞭毛已被证明是这种机会病原体的重要毒力和定居因子。我们展示了一种基因，通过生物学方法调节铜绿假单胞菌PAO1中的细菌游泳活性。然而，通过电子显微镜分析未观察到鞭毛形态的变化，这表明该基因调节了鞭毛旋转，而生物学方法无法检测到该鞭毛旋转。光子力显微镜（PFM）由欧洲分子生物学实验室的E. Stelzer首次开发，是光学镊子的高级版本，配备了可选的高灵敏度象限光电探测器（QPD），PFM能够捕获微小物体并监视物体。迅速而随机地使被困物体产生布朗波动。它具有几纳米的空间分辨率，可以在1 MHz以上的采集速率下检测探头的相对位置。通过捕获鞭毛，我们期望可以检测到鞭毛的旋转。预期铜绿假单胞菌游泳活性的研究为鞭毛在铜绿假单胞菌中的致病作用提供了有效的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号