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Dynamic and programmable cell-sorting by using microfluidics and holographic optical tweezers

机译:使用微流控和全息光镊进行动态和可编程的细胞分选

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Cell sorting is important in quantitative analysis for cellular and molecular biology study. For this purpose, we develop an opto-electro-mechanical sorting system which integrates a holographic optical tweezers (HOT) system to a microfluidics device. This system is designed to manipulate and sort various micro objects of different sizes by an optical pattern which is dynamic and programmable. In this work, we fabricate a microchannel with a main channel and two branches with polydimethylsiloxane (PDMS) which is a biocompatible elastomer material by utilizing semiconductor micro-fabrication techniques. By using a HOT system, we also generate two linear optical traps for the manipulation of micro particles. As a result, we have demonstrated that the larger particles are guided to the second branch by the two linear optical traps while the smaller particles remain unaffected and flow into the first branch of the microchannel.
机译:细胞分选在细胞和分子生物学研究的定量分析中很重要。为此,我们开发了一种光电机械分选系统,该系统将全息光镊(HOT)系统集成到微流体设备中。该系统旨在通过动态和可编程的光学图案来操纵和分类不同大小的各种微物体。在这项工作中,我们通过利用半导体微细加工技术,制造了一个具有主通道和两个分支的微通道,其中的聚二甲基硅氧烷(PDMS)是生物相容性弹性体材料。通过使用HOT系统,我们还生成了两个用于处理微粒的线性光阱。结果,我们证明了较大的颗粒被两个线性光学陷阱引导至第二分支,而较小的颗粒保持不受影响并流入微通道的第一分支。

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