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Laser microbeam - kinetic studies combined with molecule - structures reveal mechanisms of DNA repair

机译：激光微束-结合分子结构的动力学研究揭示了DNA修复的机理

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Kinetic studies on double strand DNA damages induced by a laser microbeam have allowed a precise definition of the temporal order of recruitment of repair molecules. The order is KU70 / KU80 - XRCC4 --NBS1 --RAD51. These kinetic studies are now complemented by studies on molecular structures of the repair proteins, using the program YASARA which does not only give molecular structures but also physicochemical details on forces involved in binding processes. It turns out that the earliest of these repair proteins, the KU70 / KU80 heterodimer, has a hole with high DNA affinity. The next molecule, XRCC4, has a body with two arms, the latter with extremely high DNA affinity at their ends and a binding site for Ligase 4. Together with the laser microbeam results this provides a detailed view on the early steps of DNA double strand break repair. The sequence of DNA repair events is presented as a movie.
机译：对由激光微束引起的双链DNA损伤的动力学研究已经对修复分子募集的时间顺序进行了精确定义。订单为KU70 / KU80-XRCC4 --NBS1 --RAD51。这些动力学研究现在通过使用YASARA程序对修复蛋白的分子结构进行研究来补充，该程序不仅给出分子结构，而且还给出结合过程中涉及的作用力的物理化学细节。事实证明，这些修复蛋白中最早的KU70 / KU80异二聚体具有一个具有高DNA亲和力的孔。下一个分子XRCC4，有一个带有两个臂的主体，后者的末端具有极高的DNA亲和力和Ligase 4的结合位点。再加上激光微束的结果，这提供了DNA双链早期步骤的详细视图。中断维修。 DNA修复事件的序列以电影形式呈现。

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