Fluorescence lifetime imaging using multi-dimension time-correlated single photon counting method

机译：多维时间相关单光子计数方法的荧光寿命成像

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The key technology of fluorescence lifetime imaging is introduced in this article. Time-correlated single photon counting ( TCSPC ) in Time-domain method is adopted to record fluorescence lifetime . The principle of TCSPC and thernMulti-wavelength imaging method are introduced in the paper. The counting efficiency and the amount of information in the data can be increased by recording the fluorescence in several wavelength channels simultaneously. Multi-wavelength imaging was also successfully used to separate different chromophores in stead-state images.

机译：本文介绍了荧光寿命成像的关键技术。采用时域方法中的时间相关单光子计数（TCSPC）记录荧光寿命。介绍了TCSPC的原理和多波长成像方法。通过同时在多个波长通道中记录荧光，可以提高计数效率和数据中的信息量。多波长成像还成功地用于分离稳态图像中的不同生色团。

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来源
《Optics in health care and biomedical optics IV》|2010年|p.78451V.1-78451V.5|共5页
会议地点 Beijing(CN)
作者
Cuixia SHENG; Hejing TANG;
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作者单位

College of Electrical and Electronic Engineering, Shandong University of Technology, Shandong,rnZibo, China 255049;

College of Electrical and Electronic Engineering, Shandong University of Technology, Shandong,rnZibo, China 255049;

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会议组织
原文格式 PDF
正文语种 eng
中图分类光、激光生物医学;
关键词
fluorescence lifetime; multi-dimension TCSPC; multi-wavelength imaging;

机译：荧光寿命;多维TCSPC；多波长成像;
入库时间 2022-08-26 13:44:48

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专利

1. Photon Efficiency Optimization in Time-Correlated Single Photon Counting Technique for Fluorescence Lifetime Imaging Systems [J] . Turgeman L., Fixler D. IEEE Transactions on Biomedical Engineering . 2013,第6期

机译：荧光寿命成像系统中时间相关单光子计数技术中的光子效率优化
2. A 100 fps, Time-Correlated Single-Photon-Counting-Based Fluorescence-Lifetime Imager in 130 nm CMOS [J] . IEEE Journal of Solid-State Circuits . 2014,第4期

机译：100 fps，与时间相关的基于单光子计数的130nm CMOS荧光寿命成像仪
3. Application of time-correlated single photon counting and stroboscopic detection methods with an evanescent-wave fibre-optic sensor for fluorescence-lifetime-based pH measurements [J] . Paul E Henning, Peter Geissinger Measurement Science & Technology . 2012,第4期

机译：时间相关的单光子计数和频闪检测方法与van逝波光纤传感器在基于荧光寿命的pH测量中的应用
4. Two-photon Excitation Fluorescence Lifetime Imaging Based on Time-Correlated Single Photon Counting [C] . Hejing TANG, Cuixia SHENG International Conference on Green Power, Materials and Manufacturing Technology and Applications . 2011

机译：基于时间相关的单光子计数的双光子励磁荧光寿命成像
5. ENERGY TRANSFER AMONG FLUORESCENT MOLECULES DISPERSED IN THIN FILMS OF POLYMER OR PROTEIN (RHODAMINE B, SULFORHODAMINE 101, POLYVINYL, POLY (VINYL ALCOHOL) BOVINE SERUM ALBUMIN, TIME-CORRELATED PHOTON COUNTING, LIFETIMES). [D] . SHOOP, CHRIS EDWIN. 1985

机译：荧光分子中的能量转移分散在聚合物或蛋白质（如若丹明B，磺胺二甲酰胺101，聚乙烯醇，聚乙烯醇）牛血清蛋白，稀薄的时间相关光子计数，薄膜中。
6. Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy [O] . Klaus Suhling, Liisa M. Hirvonen, Wolfgang Becker, -1

机译：基于广域时间相关的单光子计数的荧光寿命成像显微镜
7. High-Speed Wide-Field Time-Correlated Single-Photon Counting Fluorescence Lifetime Imaging Microscopy [O] . Field Ryan Michael 2014

机译：高速广域时间相关的单光子计数荧光寿命成像显微镜

Fluorescence lifetime imaging using multi-dimension time-correlated single photon counting method

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