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Determination of singlet oxygen quantum yield of HiPorfin using Singlet Oxygen Sensor Green

机译：单线态氧传感器Green测定HiPorfin的单线态氧量子产率

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摘要

Singlet oxygen (~1O-2) is widely considered to be the predominant cytotoxic agent for photodynamic therapy (PDT), and the ~1O-2 quantum yield is a key characteristic for the newly developed photosensitizers. In this study, ~1O-2 generation from the HiPorfin is monitored indirectly by using the fluorescence probe Singlet Oxygen Sensor Green (SOSG). The good linear relationship between the concentration of HiPorfin and the initial reaction rate of SOSG is observed, and the ~1O-2 quantum yield of HiPorfin is determined to be 0.124 ± 0.001, as compared to the standard reference of Rose Bengal. The results suggest that SOSG can be useful for ~1O-2 quantum yield determination for the potential photosensitizers in PDT.
机译：单线态氧（〜1O-2）被广泛认为是光动力疗法（PDT）的主要细胞毒剂，〜1O-2量子产率是新开发的光敏剂的关键特性。在这项研究中，通过使用荧光探针单重态氧传感器绿色（SOSG）间接监测了HiPorfin的〜1O-2生成。观察到HiPorfin的浓度与SOSG的初始反应速率之间具有良好的线性关系，与Rose Rosegal的标准参照物相比，HiPorfin的〜1O-2量子产率确定为0.124±0.001。结果表明，SOSG可用于确定PDT中潜在的光敏剂的〜1O-2量子产率。

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  • 来源
    《Optics in health care and biomedical optics IV》|2010年|p.78451J.1-78451J.6|共6页
  • 会议地点 Beijing(CN)
  • 作者

    Huiyun Lin; Yi Shen; Defu Chen; Lisheng Lin; Buhong Li; Shusen Xie;

  • 作者单位

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,rnFujian, China 350007;

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,rnFujian, China 350007;

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,rnFujian, China 350007;

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,rnFujian, China 350007;

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,rnFujian, China 350007;

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,rnFujian, China 350007;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 光、激光生物医学;
  • 关键词

    Photodynamic therapy; Singlet oxygen; Quantum yield; HiPorfin; Singlet Oxygen Sensor Green; Fluorescence; Initial reaction rate;

    机译：光动力疗法；单线态氧量子产率HiPorfin;单重态氧气传感器绿色；荧光;初始反应速率;
  • 入库时间 2022-08-26 13:44:48

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