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Automatic identification of various spectral features at the time-resolved excitation emission matrix of dissolved organic matters and phytoplankton cells in seawater

机译:自动识别海水中溶解的有机物和浮游植物细胞在时间分辨激发发射矩阵中的各种光谱特征

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摘要

The variation of the different parameters of the exciting radiation and the registration of the fluorescence of the investigated object allows to obtain multidimensional spectral images: from three-dimensional (length of the exciting radiation, the wavelength of the emitted radiation, the fluorescence intensity) to eight and more dimensions (in addition to three of these dimensions: spatial coordinates x, y, z; time of measurements; the duration and the intensity of the exciting radiation and etc.). In the case of measurements in natural conditions is highly desirable that the result of the processing performed during a single measurement for the operation in real time. In this paper we consider the approach described for the treatment of fluorescence measurements of dissolved organic matter and chlorophyll-a in seawater. Joint analysis of the various pairs of wavelengths of excitation / emission fluorescence, fluorescence analysis at different durations of the exciting radiation and the time-spatial analysis of the received signal allow identifying different types of fluorescent dissolved organic matter and estimate their stage of biodegradation, to study the functional state of phytoplankton cells. So it is possible to provide real-time investigation of environmental indicators of seawater.
机译:激发辐射的不同参数的变化以及所研究对象的荧光的配准可以获取多维光谱图像:从三维(激发辐射的长度,发射辐射的波长,荧光强度)到八个或更多个维度(除了其中三个维度:空间坐标x,y,z;测量时间;激发辐射的持续时间和强度等)。在自然条件下进行测量的情况下,非常需要实时进行一次测量的处理结果。在本文中,我们考虑了描述的用于处理海水中溶解性有机物和叶绿素-a荧光的方法。通过对激发/发射荧光的各种波长对进行联合分析,在激发辐射的不同持续时间进行荧光分析以及对接收信号进行时空分析,可以识别出不同类型的荧光溶解有机物,并估计它们的生物降解阶段,从而研究浮游植物细胞的功能状态。因此,可以提供对海水环境指标的实时调查。

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