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首页> 外文会议>Oral-Based Diagnostics; Annals of the New York Academy of Sciences; vol.1098 >Integrated Microfluidic Platform for Oral Diagnostics
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Integrated Microfluidic Platform for Oral Diagnostics

机译：用于口腔诊断的集成微流平台

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摘要

While many point-of-care (POC) diagnostic methods have been developed for blood-borne analytes, development of saliva-based POC diagnostics is in its infancy. We have developed a portable microfluidic device for detection of potential biomarkers of periodontal disease in saliva. The device performs rapid microfluidic chip-based immunoas-says (< 3-10 min) with low sample volume requirements (10 μL) and appreciable sensitivity (nM-pM). Our microfluidic method facilitates hands-free saliva analysis by integrating sample pretreatment (filtering, enrichment, mixing) with electrophoretic immunoassays to quickly measure analyte concentrations in minimally pretreated saliva samples. The microfluidic chip has been integrated with miniaturized electronics, optical elements, such as diode lasers, fluid-handling components, and data acquisition software to develop a portable, self-contained device. The device and methods are being tested by detecting potential biomarkers in saliva samples from patients diagnosed with periodontal disease. Our microchip-based analysis can readily be extended to detection of biomarkers of other diseases, both oral and systemic, in saliva and other oral fluids.
机译：虽然已经开发了许多针对血源性分析物的即时诊断（POC）诊断方法，但基于唾液的POC诊断的开发仍处于起步阶段。我们已经开发了一种便携式微流控设备，用于检测唾液中牙周疾病的潜在生物标记。该设备执行基于微流控芯片的快速免疫分析（<3-10分钟），样品量要求低（10μL），灵敏度高（nM-pM）。我们的微流体方法通过将样品预处理（过滤，富集，混合）与电泳免疫分析相结合，从而快速测量最少预处理的唾液样品中的分析物浓度，从而促进了免提唾液分析。该微流控芯片已与小型电子设备，光学元件（例如二极管激光器），流体处理组件以及数据采集软件集成在一起，以开发便携式的独立设备。通过检测来自诊断为牙周疾病的患者的唾液样本中的潜在生物标记物，对该设备和方法进行了测试。我们基于微芯片的分析可以轻松扩展至唾液和其他口腔液中其他疾病的生物标志物的检测，包括口腔和全身性疾病。

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