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首页> 外文会议>The papers presented at the design and nature 2014 conference >CELL CULTURE IN THREE-DIMENSIONAL LATTICES OF HYDRATED COLLAGEN FIBRILS − ITS PROGRESS AND PERSPECTIVES
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CELL CULTURE IN THREE-DIMENSIONAL LATTICES OF HYDRATED COLLAGEN FIBRILS − ITS PROGRESS AND PERSPECTIVES

机译：水合胶原蛋白纤维三维网格中的细胞培养-研究进展与展望

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摘要

Cell culture methodology has been utilized as a powerful tool to characterize cells with a presupposition that the cells in culture faithfully reproduce their in vivo phenotypes. However, it is generally recognized that the cells cultured currently by the most commonly utilized two-dimensional (2D) monolayer technology exhibit different phenotypes from those in living tissues. Cultivation of cells in three-dimensional (3D) lattices of collagen fibrils (collagen gel culture) has been thought to overcome the shortages of 2D cell culture such that the cells behave as in vivo by interacting with not only nearby other cells but also by surrounding extracellular matrices. A remarkable outcome of studies on collagen gel culture was a demonstration of contractile nature of the cells. One of the not-fully appreciated issues about the 3D cell culture is the effect of fluid flow through the collagen gels on cells’ phenotypes. In this review, we make a short overview of historical and current studies of the collagen gel culture from a viewpoint of ‘how we can more correctly extract the cell-related phenomena in living tissues/organs by in vitro culture technology, placing an emphasis on the importance of stimuli caused by flow of cell culture medium.’
机译：细胞培养方法已被用作表征细胞的强大工具，其前提是培养中的细胞能够忠实地再现其体内表型。然而，通常认识到，目前通过最常用的二维（2D）单层技术培养的细胞表现出与活组织不同的表型。人们认为，在胶原纤维的三维（3D）晶格中进行细胞培养（胶原凝胶培养）可以克服2D细胞培养的不足，即细胞不仅与附近的其他细胞相互作用，而且与周围的细胞相互作用，因此具有体内作用细胞外基质。胶原蛋白凝胶培养研究的显著成果是细胞收缩性质的证明。关于3D细胞培养的一个尚未完全意识到的问题之一是流体流过胶原蛋白凝胶对细胞表型的影响。在这篇综述中，我们从“我们如何通过体外培养技术更正确地提取活组织/器官中的细胞相关现象的观点”的观点对胶原凝胶培养的历史和当前研究作了简短的概述，重点是细胞培养基流动引起的刺激的重要性。”

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