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Variable DIC system for anisotropic and submicron objects

机译:各向异性和亚微米物体的可变DIC系统

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Abstract: Nomarski's DIC system for transmitted light consists of two birefringent prisms, one of which is located behind the microscope objective, while the other is placed below the condenser. The latter is used as a compensator; it is similar to the former and oriented in such a manner as to cancel out the optical path differences between lightwave components slightly sheared and brought into mutual interference. Due to the sub- condenser birefringent prism, this system suffers from some limitations when anisotropic objects are examined. These limitations can be removed if a subtractive combination of two birefringent prisms is placed behind the microscope objective and the sub-condenser prism is replaced by a slit diaphragm. If the required wavefront shear is equal to or less than the resolving power of the objective (this is the case of submicron objects), then the slit of the sub-condenser diaphragm is very large or even equal to the diameter of the condenser aperture. Moreover, a variable wavefront can be achieved easily by a suitable combination of two birefringent prisms installed behind the microscope objective. !5
机译:摘要:Nomarski的透射光DIC系统由两个双折射棱镜组成,其中一个位于显微镜物镜后面,而另一个位于聚光镜下方。后者用作补偿器。它类似于前者,其取向是抵消了略微剪切并引起相互干扰的光波分量之间的光程差。由于次聚光镜是双折射棱镜,因此在检查各向异性物体时,该系统会受到一些限制。如果将两个双折射棱镜的减法组合放置在显微镜物镜的后面,并且将副聚光棱镜替换为狭缝光阑,则可以消除这些限制。如果所需的波前剪切力等于或小于物镜的分辨能力(这是亚微米物体的情况),则子聚光镜的缝隙非常大,甚至等于聚光镜孔径的直径。此外,通过适当地安装在显微镜物镜后面的两个双折射棱镜可以轻松实现可变的波前。 !5

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