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首页> 外文会议>Photodynamic Therapy of Cancer >Kinetics of the photodynamic damage of phthalocyanine-loaded cultured K562 cells studied by means of flow cytometry
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Kinetics of the photodynamic damage of phthalocyanine-loaded cultured K562 cells studied by means of flow cytometry

机译：流式细胞术研究酞菁培养的K562细胞光动力损伤的动力学

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Abstract: The kinetics of cellular damage after photodynamic action was studied by means of flow-cytometry. Cultured K562 cells were loaded with tetrasulphonated aluminum phthalocyanine and propidium iodide, acridine orange and rhodamine 123 were used to assess the damage done to the cells. Exposure of phthalocyanine-loaded cells to light resulted in the following order of events: increase of the intracellular Pc fluorescence $MIN$GRT mitochondrial damage $MIN$GRT changes of the cell surface and/or volume $MIN$GRT plasma membrane potential vanishing $MIN$GRT severe damage of cell plasma membrane. For all these processes it takes approximately two hours to reach the stationary state. !17
机译：摘要：利用流式细胞仪研究了光动力作用后细胞损伤的动力学。在培养的K562细胞中加入四磺酸铝酞菁，并使用碘化丙啶，a啶橙和若丹明123评估对细胞的损伤。酞菁负载的细胞暴露于光导致以下事件顺序：细胞内Pc荧光增加$ MIN $ GRT线粒体损伤$ MIN $ GRT细胞表面和/或体积的变化$ MIN $ GRT质膜电位消失$ MIN $ GRT对细胞质膜的严重破坏。对于所有这些过程，大约需要两个小时才能达到静止状态。 ！17

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