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首页> 外文会议>Photon Migration and Imaging in Random Media and Tissues >Multiple multipole scattering of light in mitochondrial suspensions
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Multiple multipole scattering of light in mitochondrial suspensions

机译：线粒体悬浮液中光的多重多极散射

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Abstract: In a recent optical study two dimensional intensity patterns of light irradiated into suspensions of mitochondria were investigated. We designed our experimental model in such a way that the concentration of scattering particles was similar to concentrations found in tissues of humans and mammals. Earlier measurements performed in isolated and hemoglobin-free perfused rat liver and heart revealed that tissue spectra were strongly altered by light scattering when functional states of the two organs e.g. tissue oxygenation were changed. Based upon Mie's theory as well as our observations in perfused organs we concluded that an important factor able to induce sizeable changes in light scattering under physiological or pathophysiological conditions could be alterations in size of subcellular particles. The classical Mie theory which yields for single multipole scattering shows an increase in forward and backward scattering as a function of particle radius. E.g. when particle size is increased from a radius of 0.1 to 2 $mu@m forward scattering is augmented by a factor of 273,000 while backward scattering attains a value of 13,900. Measurements of angular light distribution obtained in mitochondrial suspensions revealed first evidence that multiple multipole scattering increases backward scattering while forward scattering becomes diminished when particle size is enlarged. !3
机译：摘要：在最近的光学研究中，研究了照射到线粒体悬浮液中的二维光强度模式。我们设计实验模型的方式是使散射粒子的浓度类似于在人类和哺乳动物组织中发现的浓度。在离体且无血红蛋白的灌注大鼠肝脏和心脏中进行的较早测量显示，当两个器官的功能状态（例如血脂，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白，血红蛋白和血红蛋白）大大改变时，组织光谱会发生很大的变化。组织氧合改变。基于米氏理论以及我们在灌注器官中的观察，我们得出结论，在生理或病理生理条件下能够引起光散射发生较大变化的重要因素可能是亚细胞颗粒大小的改变。产生单多极散射的经典Mie理论表明，前向和后向散射随粒子半径的增加而增加。例如。当粒径从0.1的半径增加到2μm时，前向散射增加了273,000倍，而后向散射达到了13,900。在线粒体悬浮液中获得的角光分布的测量揭示了第一个证据，即当粒径增大时，多极散射增加了后向散射，而前向散射却减小了。 ！3

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