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首页> 外文会议>Photonic Therapeutics and Diagnostics II; Progress in Biomedical Optics and Imaging; vol.7, no.1 >Multi-photon microscopy of tobacco-exposed organotypic skin models
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Multi-photon microscopy of tobacco-exposed organotypic skin models

机译：烟草暴露器官型皮肤模型的多光子显微镜

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摘要

Cigarette smoking is the most preventable cause of death in the United States. Researchers have extensively studied smoking in regards to its association with cancer, cardiovascular, and pulmonary disease. In contrast, the impact of cigarette smoking on skin has received much less attention. To provide a better understanding of the effect of cigarette smoking on the human dermal layer, this study used multi-photon microscopy (MPM) to examine collagen in organotypic skin models exposed to cigarette smoke condensate (CSC). Adult and neonatal organotypic tissue-engineered artificial skin models (RAFTs) were constructed and exposed to varying concentrations of CSC. Imaging of the RAFTs was performed using MPM and second-harmonic generation signals (SHG), which allowed for collagen structure to be viewed and analyzed as well as for collagen density to be assessed from derived depth-dependent decay (DDD) values. RAFT contraction as related to exposure concentration was monitored as well. Results indicated a dose dependent between contraction rates and CSC concentration. Collagen structure showed more preservation of its original structure at a greater depth in RAFTs with higher concentrations of CSC. No clear trends could be drawn from analysis of derived DDD values.
机译：在美国，吸烟是最可预防的死亡原因。研究人员已经广泛研究了吸烟与癌症，心血管和肺部疾病的关系。相反，吸烟对皮肤的影响却很少受到关注。为了更好地了解吸烟对人类真皮层的影响，本研究使用多光子显微镜（MPM）来检查暴露于香烟冷凝物（CSC）的器官型皮肤模型中的胶原蛋白。构建成人和新生儿器官型组织工程人工皮肤模型（RAFT），并将其暴露于不同浓度的CSC。 RAFT的成像是使用MPM和二次谐波生成信号（SHG）进行的，可以查看和分析胶原结构，并可以根据衍生的深度相关衰减（DDD）值评估胶原密度。还监测了与暴露浓度有关的RAFT收缩。结果表明剂量取决于收缩率和CSC浓度。胶原蛋白结构在具有较高CSC浓度的RAFT中显示出更深的原始结构保存。从导出的DDD值的分析中无法得出明确的趋势。

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