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Fluorescence of the bladder washout fluid following cystoscopy: a preliminary study

机译：膀胱镜检查后膀胱冲洗液的荧光：初步研究

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During fluorescence cystoscopy, it is observed that the acquired images are sometimes blurred by a greenish background originating from the bladder washout fluid. Several fluorophores are involved in this overall liquid fluorescence, and their exact origin and relative contributions remain unknown. In this study, the bladder washout fluid is sampled at different times during fluorescence cystoscopy examinations. In total, 32 samples from 12 patients are analyzed with a spectrofluorimeter (excitation range: 350-445 nm, emission range 380-700 nm). This study shows clearly that the position of the fluorescence peaks (excitation/emission wavelengths: 450/525 nm, 405/625 nm) and shoulder (440/525 nm) is reproducible between different patients. It also suggests that an excitation at wavelengths higher than 400 nm helps to suppress this solution background fluorescence. Additionally, the pH of the solution seems to influence the position of the fluorescence peaks, and this suggests that changing the pH of the examination liquid could help in avoiding the greenish background.
机译：在荧光膀胱镜检查期间，观察到的是，有时采集的图像会因来自膀胱冲洗液的绿色背景而变得模糊。几种荧光团参与了整个液体荧光，它们的确切来源和相对贡献仍然未知。在这项研究中，在荧光膀胱镜检查期间的不同时间取样膀胱冲洗液。使用分光荧光计（激发范围：350-445 nm，发射范围380-700 nm）对来自12位患者的32个样品进行了分析。这项研究清楚地表明，不同患者之间的荧光峰（激发/发射波长：450/525 nm，405/625 nm）和肩部（440/525 nm）的位置可重现。这也表明，在高于400 nm的波长处进行激发有助于抑制该溶液的背景荧光。此外，溶液的pH值似乎会影响荧光峰的位置，这表明更改检查液的pH值可有助于避免绿色背景。

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