• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Photonic therapeutics and diagnostics X >Raman spectroscopy and oral exfoliative cytology
【24h】

Raman spectroscopy and oral exfoliative cytology

机译：拉曼光谱和口腔脱落细胞学

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Early detection of oral cancers can substantially improve disease-free survival rates. Ex vivo and in vivo Raman spectroscopic (RS) studies on oral cancer have demonstrated the applicability of RS in identifying not only malignant and premalignant conditions but also cancer-field-effects: the earliest events in oral carcinogenesis. RS has also been explored for cervical exfoliated cells analysis3. Exfoliated cells are associated with several advantages like non-invasive sampling, higher patient compliance, transportation and analysis at a central facility: obviating need for on-site instrumentation. Thus, oral exfoliative cytology coupled with RS may serve as a useful adjunct for oral cancer screening. In this study, exfoliated cells from healthy controls with and without tobacco habits, premalignant lesions (leukoplakia and tobacco-pouch-keratosis) and their contralateral mucosa were collected using a Cytobrush. Cells were harvested by vortexing and centrifugation at 6000 rpm. The cellular yield was ascertained using Neubauer's chamber. Cell pellets were placed on a CaF_2 window and Raman spectra were acquired using a Raman microprobe (40X objective) coupled HE-785 Raman spectrometer. Approximately 7 spectra were recorded from each pellet, following which pellet was smeared onto a glass slide, fixed in 95% ethanol and subjected to Pap staining for cytological diagnosis (gold standard). Preliminary PC-LDA followed by leave-one-out cross validation indicate delineation of cells from healthy and all pathological conditions. A tendency of classification was also seen between cells from contralateral, healthy tobacco and site of premalignant lesions. These results will be validated by cytological findings, which will serve as the basis for building standard models of each condition.
机译：早期发现口腔癌可以大大提高无病生存率。口腔癌的离体和体内拉曼光谱（RS）研究表明，RS不仅适用于识别恶性和恶性疾病，而且还可以识别癌场效应：口腔癌发生的最早事件。 RS也已被用于宫颈脱落细胞分析3。脱落的细胞具有多种优势，例如无创采样，更高的患者依从性，中央设施的运输和分析：无需现场仪器。因此，与RS结合的口腔剥脱性细胞学可以作为口腔癌筛查的有用辅助手段。在这项研究中，使用Cytobrush收集了有和没有烟草习惯，恶变前病变（白斑和烟草袋角化病）及其对侧粘膜的健康对照的脱落细胞。通过涡旋并以6000 rpm离心收集细胞。使用Neubauer室确定细胞产量。将细胞沉淀置于CaF_2窗口上，并使用拉曼显微探针（40X物镜）耦合HE-785拉曼光谱仪获得拉曼光谱。从每个沉淀中记录大约7个光谱，然后将沉淀涂在载玻片上，固定在95％乙醇中，并进行Pap染色以进行细胞学诊断（金标准）。初步的PC-LDA，然后进行留一法交叉验证，表明了健康和所有病理状况下细胞的轮廓。对侧健康烟草细胞和癌前病变部位之间也发现了分类的趋势。这些结果将通过细胞学发现得到验证，这将为建立每种疾病的标准模型奠定基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号