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首页> 外文会议>Photosynthesis research for food, fuel and future >Compositional and Structural Analyses of the Photosystem Ⅱ Isolated from the Red Alga Cyanidioschyzon Merolae
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Compositional and Structural Analyses of the Photosystem Ⅱ Isolated from the Red Alga Cyanidioschyzon Merolae

机译：红藻Cyanidioschyzon Merolae分离的光系统Ⅱ的组成和结构分析。

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摘要

Members of the rhodophytan order Cyanidiales are unique among phototrophs in their ability to live in extremely low pH levels and moderately high temperatures. The photosynthetic apparatus of the red alga Cyanidioschyzon merolae represents an intermediate type between cyanobacteria and higher plants, suggesting that this alga may provide the evolutionary link between prokaryotic and eukaryotic phototrophs. While red algal PSI resembles that of the higher plants, the PSⅡ complex is more reminiscent of the cyanobacterial ancestor in that it contains phycobilisomes as the light-harvesting system instead of Chla/b binding proteins of green algae and higher plants, as well as the PsbU and PsbV subunits stabilising the oxygen evolving complex (OEC). The most remarkable feature of the red algal PSⅡ is the presence of the fourth extrinsic protein of 20 kDa (PsbQ') which is not found in the cyanobacterial OEC and which is distantly related with the green algal PsbQ. This feature together with some differences in the structural cooperation between the OEC subunits suggests that the lumenal side of red algal PSⅡ may vary from the prokaryotic ancestor. In order to elucidate the structural differences between cyanobacterial and eukaryotic PSⅡ, we have isolated highly active and stable dimeric complexes of the C. merolae PSⅡ and subjected them to high throughput crystallization and mass spectrometry analyses. Here we report the full subunit composition and preliminary results of 3D crystallization of the dimeric C. merolae PSⅡ.
机译：红藻丹级蓝藻的成员在光养生物中是独一无二的，因为它们能够生活在极低的pH水平和适度的高温下。红藻Cyanidioschyzon merolae的光合作用代表蓝藻和高等植物之间的中间类型，这表明该藻类可能提供原核和真核生物之间的进化联系。虽然红色藻类PSI与高等植物相似，但PSⅡ复合物更让人联想到蓝细菌祖先，因为它含有藻胆体作为光捕获系统，而不是绿藻和高等植物以及植物的Chla / b结合蛋白。 PsbU和PsbV亚基可稳定氧气释放复合物（OEC）。红色藻类PSⅡ的最显着特征是存在20 kDa的第四个外在蛋白（PsbQ'），该蛋白在蓝藻OEC中没有发现，并且与绿色藻类PsbQ密切相关。该特征以及OEC亚基之间的结构协作上的一些差异表明，红色藻类PSⅡ的管腔侧可能与原核祖先不同。为了阐明蓝细菌和真核PSⅡ之间的结构差异，我们分离了C. merolaePSⅡ的高活性和稳定二聚配合物，并对其进行了高通量结晶和质谱分析。在这里我们报告了二聚体C. merolaePSⅡ的完整亚基组成和3D结晶的初步结果。

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