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首页> 外文会议>Photosynthesis research for food, fuel and future >Involvement of slr0081, a Two-Component Signal-Transduction System Response Regulator,in Acid Stress Tolerance in Synechocystis sp. PCC 6803
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Involvement of slr0081, a Two-Component Signal-Transduction System Response Regulator,in Acid Stress Tolerance in Synechocystis sp. PCC 6803

机译：二组分信号传导系统响应调节剂slr0081参与耐蓝藻的耐酸胁迫。 PCC 6803

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摘要

Two-component signal transduction is the primary signaling mechanism used for global regulation of cell response to changes in the environment. DNA microarray analysis identified genes up-regulated by acid stress in cyanobacteria Synechocystis sp. PCC 6803. Several of these altered genes are thought to be response regulators that are directly involved in this type of stress. Deletion mutants of response regulator genes were constructed and survivability was compared between the cells transfected with mutant and wild-type genes in a low-pH medium. Among these, deletion of slr0081 affected the growth rate under conditions of acid stress (pH 6.0). We examined the genome-wide expression of genes in Aslr0081 mutant cells by using DNA microarray in an attempt to determine whether slr0081 is involved in the regulation of other acid stress responsive genes. Our findings by quantitative real-time RT PCR revealed that down regulation of acid responsive genes slr0967 and sll0939 occurs by deletion of slr0081.
机译：两成分信号转导是用于整体调节细胞对环境变化的反应的主要信号传导机制。 DNA微阵列分析确定了蓝藻Synechocystis sp。中受酸胁迫上调的基因。 PCC6803。这些改变的基因中有几个被认为是直接参与这种类型压力的反应调节剂。构建了应答调节基因的缺失突变体，并在低pH培养基中比较了用突变型基因和野生型基因转染的细胞之间的存活率。其中，slr0081的缺失影响了酸胁迫（pH 6.0）条件下的生长速率。我们通过使用DNA芯片检查了Aslr0081突变细胞中基因的全基因组表达，以试图确定slr0081是否参与其他酸胁迫响应基因的调控。我们通过定量实时RT PCR的发现表明，酸响应基因slr0967和sll0939的下调是通过删除slr0081来实现的。

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  • 来源
  • 会议地点 Beijing(CN)
  • 作者

    Yu Tanaka; Mayuko Kimura; Atsushi Moriyama; Yuko Kubo; Mamoru Sambe; Junji Uchiyama; Hisataka Ohta;

  • 作者单位

    Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, Japan;

    Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, Japan;

    Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, Japan;

    Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, Japan;

    Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, Japan;

    Research center for RNA science, RIST, Tokyo University of Science, Noda, Chiba 278-8510, Japan;

    Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, Japan Research center for RNA science, RIST, Tokyo University of Science, Noda, Chiba 278-8510, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 光合作用、碳素同化作用;
  • 关键词

    Low-pH; Cyanobacteria; Stress response; Two-component system;

    机译：低pH;蓝细菌;压力反应;两组分体系;
  • 入库时间 2022-08-26 14:02:16

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