Psbo Degradation by Deg Proteases under Reducing Conditions

机译：还原条件下Deg蛋白酶对Psbo的降解

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DegP/HtrA proteases are ATP-independent serine endopeptidases widely distributed in nearly all organisms. As yet, their physiological role in oxygenic photosynthetic organisms is unclear, although it has been widely speculated that they participate in the Photosystem Ⅱ repair cycle. Here, we investigated the ability of Deg proteases to degrade PsbO according to its redox state. A sample of purified PsbO or Photosystem Ⅱ complex was incubated together with recombinant Deg proteases of Synechocystis sp. PCC 6803 (HhoA, HhoB or HtrA). The reducing media was conferred by the Escherichia coli thioredoxin/thioredoxin reductase system. The results obtained showed that HhoA is able to hydrolyze reduced PsbO while HhoB and HtrA are not. HhoA was active against free PsbO of spinach as well as PsbO of Synechocystis attached to Photosystem Ⅱ, only under reducing conditions. The finding that all three Deg proteases of Synechocystis co-purify with Photosystem Ⅱ supports the hypothesis of PsbO as a substrate for Deg proteases in vivo.

机译：DegP / HtrA蛋白酶是不依赖ATP的丝氨酸内肽酶，广泛分布于几乎所有生物中。到目前为止，还不清楚它们在含氧光合生物中的生理作用，尽管人们普遍认为它们参与了PhotosystemⅡ的修复周期。在这里，我们研究了Deg蛋白酶根据其氧化还原状态降解PsbO的能力。将纯化的PsbO或PhotosystemⅡ复合物样品与集胞藻（Syechochocystis sp）的重组Deg蛋白酶一起孵育。 PCC 6803（HhoA，HhoB或HtrA）。还原培养基由大肠杆菌硫氧还蛋白/硫氧还蛋白还原酶系统赋予。获得的结果表明，HhoA能够水解还原的PsbO，而HhoB和HtrA则不能。 HhoA仅在还原条件下对菠菜的游离PsbO以及与光系统Ⅱ相连的集胞藻的PsbO具有活性。集胞藻的三种Deg蛋白酶与PhotosystemⅡ共纯化的发现支持了PsbO作为体内Deg蛋白酶底物的假说。

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来源
《Photosynthesis research for food, fuel and future》|2010年|599-602|共4页
会议地点 Beijing(CN)
作者
Irma N Roberts; Helder Miranda; L(a)m Xu(a)n T(a)m; Thomas Kieselbach; Christiane Funk;
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Department of Chemistry and Ume(a)Plant Science Centre, Ume(a) University, SE - 901 87 Ume(a), Sweden;

Department of Chemistry and Ume(a) Plant Science Centre, Ume(a) University, SE - 901 87 Ume(a), Sweden;

Department of Chemistry and Ume(a) Plant Science Centre, Ume(a) University, SE - 901 87 Ume(a), Sweden;

Department of Chemistry and Ume(a) Plant Science Centre, Ume(a) University, SE - 901 87 Ume(a), Sweden;

Department of Chemistry and Ume(a) Plant Science Centre, Ume(a) University, SE - 901 87 Ume(a), Sweden;

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原文格式 PDF
正文语种 eng
中图分类光合作用、碳素同化作用;
关键词
Deg proteases; PsbO; Protein degradation; Thioredoxin; Synechocystis; Sp. PCC 6803;

机译：Deg蛋白酶; PsbO;蛋白质降解;硫氧还蛋白; Synechocystis; Sp。 PCC 6803;

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专利

1. Deg1 is involved in the degradation of the PsbO oxygen-evolving protein of photosystem II in Arabidopsis [J] . LI Jin, SUN XuWu, ZHANG LiXin 中国科学通报：英文版 . 2010,第027期

机译：Deg1参与拟南芥中光系统II的PsbO释氧蛋白的降解。
2. Synergic Effect of Bacterial Glycosidases and Digestive Proteases on Mucus Degradation and the Reduced Risk of Inflammatory Bowel Disease-like Gut Damage in Both Germ-free and Poor Hygiene Conditions [J] . Liliana Bordeianou Inflammatory bowel diseases . 2008,第1期

机译：细菌糖苷酶和消化蛋白酶对粘液菌和疾病疾病样肠状肠道损伤降低风险的协同作用
3. Lumenal exposed regions of the D1 protein of PSII are long enough to be degraded by the chloroplast Deg1 protease [J] . Ronit Rimon Knopf, Zach Adam Scientific reports. . 2018,第1期

机译：PSII的D1蛋白的腔外暴露区域足够长，可以通过叶绿体DEG1蛋白酶降解
4. Charge accumulation in LDPE and XLPE conditioned at 80/spl deg/C under reduced pressure [C] . Fleming, R.J., Henriksen, Electrical Insulation and Dielectric Phenomena, 1997. IEEE 1997 Annual Report., Conference on . 1997

机译：LDPE和XLPE中在80 / spl deg / C的减压条件下积累的电荷
5. Sulfate reduction at -0.5°C under halophilic and anaerobic conditions by sulfate-reducing bacteria. [D] . Cannon, Bailey K. 2006

机译：在嗜盐和厌氧条件下，通过还原硫酸盐的细菌在-0.5°C下还原硫酸盐。
6. Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent [O] . Irma N. Roberts, Xuan Tam Lam, Helder Miranda, -1

机译：psbO的降解的DEG蛋白酶Hhoa是硫氧还蛋白依赖
7. Degradation of PsbO by the deg protease HhoA is thioredoxin dependent [O] . Roberts, Irma N, Lam, Xuan Tam, Miranda, Helder, 2012

机译：降解蛋白酶HhoA对PsbO的降解是硫氧还蛋白依赖性的

Psbo Degradation by Deg Proteases under Reducing Conditions

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