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Effects of 1-butanol, Neomycin, and Calcium on the Photosynthetic Characteristics of pepc Transgenic Rice

机译:1-丁醇,新霉素和钙对pepc转基因水稻光合特性的影响

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The effects of 1-butanol, neomycin, and calcium on the photosynthesis of pepc transgenic rice were studied with over-expressed pepc transgenic rice (PC) of the 8th generation as study materials and with non-transgenic wild type (WT) rice and maize, a typical C4 plant, as control. The results indicated that 0.04% 1-butanol, 0.1 mmol L-1 neomycin, and 0.05 mmol IT-1 calcium ions had not a significant effect on the net photosynthetic rate (Pn) of the three tested materials. The stepwise multiple linear regression analysis showed that the pepc transgenic rice maintained its relative high net photosynthesis rate through increasing stomatal conductance. It was proved by the scanning electron microscope (SEM) that compared with the WT, the stomatal density of PC leaves increased while the stomatal aperture also increased under the 1-butanol treatment. The separate 1-butanol treatment significantly inhibited the PEPC activity of PC and maize leaves, while neomycin treatment alone had no significant effects on the PC and maize. However, the combined treatment with 1butanol and neomycin evidently promoted the PEPC activity of PC and WT. Moreover, exogenous calcium significantly promoted the PEPC activity of the three materials. It is clear that the exogenous regulation on PEPC enzyme of pepc transgenic rice is different from both C4 and C3 plants.
机译:以第8代过表达的pepc转基因水稻(PC)为研究材料,并以非转基因野生型(WT)水稻和玉米研究1-丁醇,新霉素和钙对pepc转基因水稻光合作用的影响,作为典型的C4工厂。结果表明,0.04%1-丁醇,0.1 mmol L-1新霉素和0.05 mmol IT-1钙离子对三种测试材料的净光合速率(Pn)没有显着影响。逐步多元线性回归分析表明,pepc转基因水稻通过增加气孔导度来维持其较高的净光合作用速率。扫描电子显微镜(SEM)证明,与WT相比,在1-丁醇处理下,PC叶的气孔密度增加,而气孔孔径也增加。单独的1-丁醇处理显着抑制了PC和玉米叶片的PEPC活性,而单独使用新霉素对PC和玉米没有显着影响。然而,与1-丁醇和新霉素的联合处理明显促进了PC和WT的PEPC活性。此外,外源钙显着促进了这三种材料的PEPC活性。显然,pepc转基因水稻对PEPC酶的外源调控与C4和C3植物均不同。

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