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首页> 外文会议>PIAGENG 2013: intelligent information, control, and communication technology for agricultural engineering >Genetic diversity of phytophthoRA sojae isolates in Heilongjiang province in China assessed by RAPD and EST-SSR
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Genetic diversity of phytophthoRA sojae isolates in Heilongjiang province in China assessed by RAPD and EST-SSR

机译：应用RAPD和EST-SSR技术评估中国黑龙江省大豆疫霉菌的遗传多样性

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摘要

Random-amplified polymorphic DNA (RAPD) and EST-SSR markers were used to estimate the genetic relationship among thirty-nine P.sojae isolates from three locations in Heilongjiang Province, and nine isolates from Ohio in America were made as reference strains. 10 of 50 RAPD primers and 5 of 33 EST-SSR were polymorphic across 48 P.sojae isolates. Similarity values among P.sojae isolates were from 49% to 82% based on the RAPD data. The similarities based on EST-SSR markers ranged from 47% to 85%. The genetic diversity revealed by EST-SSR marker analysis was higher than that obtained from RAPD. The similarity matrices for the SSR data and the RAPD data were moderately correlated (r = 0.47). Genetic similarity coefficients were also relatively lower, which demonstrated complicated genetic background within each location. The high similarity values range revealed the ability of RAPD/EST-SSR markers to distinguish even among morphological similar phytophthora.
机译：利用随机扩增多态性DNA（RAPD）和EST-SSR标记来估计黑龙江省三个地方的三十九株大豆疫霉菌的遗传关系，并以美国俄亥俄州的九株为参考菌株。 50个RAPD引物中的10个和33个EST-SSR中的5个在48个大豆疫霉分离株中具有多态性。基于RAPD数据，大豆假单胞菌分离株之间的相似度值从49％到82％。基于EST-SSR标记的相似性范围为47％至85％。 EST-SSR标记分析揭示的遗传多样性高于RAPD。 SSR数据和RAPD数据的相似度矩阵适度相关（r = 0.47）。遗传相似性系数也相对较低，这表明每个位置的遗传背景复杂。高相似值范围揭示了RAPD / EST-SSR标记甚至在形态相似的疫霉菌之间也具有区分能力。

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  • 会议地点 Sanya(CN)
  • 作者

    J. J. Wu; P. F. Xu; L. J. Liu; J. S. Wang; W. G. Lin; S. Z. Zhang; L. Wei;

  • 作者单位

    Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, China 150086 Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086;

    Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, China 150030;

    Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086;

    Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086;

    Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086;

    Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, China 150030;

    Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086;

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  • 正文语种 eng
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  • 关键词

    Genetic diversity; Phytophthora sojae; RAPD; EST-SSR; Cluster analysis;

    机译：遗传多样性;大豆疫霉菌； RAPD； EST-SSR；聚类分析;

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