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Plasmonic cell manipulation for biomedical and screening applications

机译：用于生物医学和筛选应用的血浆细胞操纵

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摘要

Modulation of the cell membrane permeability by the plasmonic interaction of gold nanoparticles and short laser pulses for cell manipulation or destruction has been the objective of several recent studies. Gold nanoparticles in close vicinity to the cellular membrane are irradiated to evoke a nanoscale membrane perforation, enabling extracellular molecules to enter the cell. However, besides several basic studies no real translation from proof of concept experiments to routine usage of this approach was achieved so far. In order to provide a reproducible and easy-to-use platform for gold nanoparticle mediated (GNOME) laser manipulation, we established an automated and encased laser setup. We demonstrate its feasibility for high-throughput cell manipulation. In particular, protein delivery into canine cancer cells is shown. The biofunctional modification of cells was investigated using the caspase 3 protein, which represents a central effector molecule in the apoptotic signaling cascade. An efficient and temporally well-defined induction of apoptosis was observed with an early onset 2 h after protein delivery by GNOME laser manipulation. Besides protein delivery, modulation of gene function using GNOME laser transfection of antisense molecules was demonstrated, showing the potential of this technique for basic science and screening purposes. Concluding we established GNOME laser manipulation of cells as a routine method, which can be utilized reliably for the efficient delivery of biomolecules. Its intrinsic features, being low impairment of the cell viability, high delivery efficiency and universal applicability, render this method well suited for a large variety of biomedical application.
机译：金纳米颗粒和短激光脉冲的等离子体相互作用对细胞膜通透性的调控，已成为一些近期研究的目标。辐照细胞膜附近的金纳米颗粒，以引起纳米级膜穿孔，使细胞外分子进入细胞。但是，到目前为止，除了一些基础研究之外，还没有从概念验证实验到常规使用这种方法的真正翻译。为了为金纳米粒子介导的（GNOME）激光操作提供可重现且易于使用的平台，我们建立了自动化的封装激光设置。我们证明了其高通量细胞操作的可行性。特别地，显示了蛋白质递送到犬癌细胞中。使用caspase 3蛋白研究了细胞的生物功能修饰，该蛋白代表凋亡信号级联反应中的中心效应分子。在通过GNOME激光操纵蛋白质递送后的2 h早期，观察到了有效的和时间明确的凋亡诱导。除蛋白质递送外，还证明了使用GNOME反义分子激光转染对基因功能的调节，显示了该技术在基础科学和筛选目的方面的潜力。最后，我们建立了GNOME激光对细胞进行操作的常规方法，该方法可以可靠地用于有效传递生物分子。它的固有特征是对细胞存活率的低损害，高递送效率和普遍适用性，使得该方法非常适合于多种生物医学应用。

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  • 来源
    《Plasmonics in biology and medicine XII》|2015年|93400Q.1-93400Q.5|共5页
  • 会议地点 San Francisco CA(US)
  • 作者

    Dag Heinemann; Markus Schomaker; Stefan Kalies; Merve Sinram; Patrick Heeger; Hugo Murua Escobar; Heiko Meyer; Tammo Ripken;

  • 作者单位

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

    Division of Medicine Clinic Ⅲ, Hematology, Oncology and Palliative Medicine, University of Rostock, Ernst-Heydemann-Str. 6, 18057 Rostock, Germany;

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

    Biomedical Optics Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany;

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  • 正文语种 eng
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  • 关键词

    Laser transfection; protein delivery; apoptosis; plasmonics;

    机译：激光转染；蛋白质输送；细胞凋亡等离子体;

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