• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Plasmonics in biology and medicine XIII >Plasmonic Nanostructures for Bioanalytical Applications of SERS
【24h】

Plasmonic Nanostructures for Bioanalytical Applications of SERS

机译：SERS生物分析应用的等离子纳米结构。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Surface-enhanced Raman scattering (SERS) is a potential analytical technique for the detection and identification of chemicals and biological molecules and structures in the close vicinity of metallic nanostructures. We present a novel method to fabricate tunable plasmonic nanostructures and perform a comprehensive structural and optical characterization of the structures. Spherical latex particles are uniformly deposited on glass slides and used as templates to obtain nanovoid structures on polydimethylsiloxane surfaces. The diameter and depth of the nanovoids are controlled by the size of the latex particles. The nanovoids are coated with a thin Ag layer for fabrication of uniform plasmonic nanostructures. Structural characterization of the surfaces is performed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Optical properties of these plasmonic nanostructures are evaluated via UV/Vis spectroscopy, and SERS. The sample preparation step is the key point to obtain strong and reproducible SERS spectra from the biological structures. When the colloidal suspension is used as a SERS substrate for the protein detection, the electrostatic interaction of the proteins with the nanoparticles is described by the nature of their charge status, which influences the aggregation properties such as the size and shape of the aggregates, which is critical for the SERS experiment. However, when the solid SERS substrates are fabricated, SERS signal of the proteins that are background free and independent of the protein charge. Pros and cons of using plasmonic nano colloids and nanostructures as SERS substrate will be discussed for label-free detection of proteins using SERS.
机译：表面增强拉曼散射（SERS）是一种潜在的分析技术，用于检测和识别金属纳米结构附近的化学物质，生物分子和结构。我们提出了一种新颖的方法来制造可调谐等离子体纳米结构，并对结构进行全面的结构和光学表征。球形胶乳颗粒均匀地沉积在载玻片上，并用作模板以在聚二甲基硅氧烷表面上获得纳米空隙结构。纳米空隙的直径和深度由乳胶颗粒的大小控制。纳米空隙涂覆有薄的Ag层，用于制造均匀的等离子体纳米结构。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）对表面进行结构表征。这些等离激元纳米结构的光学性质通过紫外/可见光谱和SERS评估。样品制备步骤是从生物结构中获得强大且可重现的SERS光谱的关键。当将胶体悬浮液用作蛋白质检测的SERS底物时，蛋白质与纳米颗粒的静电相互作用由其电荷状态的性质来描述，这会影响聚集性质，例如聚集体的大小和形状，对于SERS实验至关重要。但是，当制造固态SERS底物时，无背景且与蛋白质电荷无关的蛋白质的SERS信号。将讨论使用等离激元纳米胶体和纳米结构作为SERS底物的利弊，以便使用SERS对蛋白质进行无标记检测。

著录项

  • 来源
    《Plasmonics in biology and medicine XIII》|2016年|972405.1-972405.7|共7页
  • 会议地点 San Francisco CA(US)
  • 作者

    Mehmet KAHRAMAN; Sebastian WACHSMANN-HOGIU;

  • 作者单位

    Department of Chemistry, Faculty of Arts and Sciences, University of Gaziantep, 27310 Sehitkamil, Gaziantep, Turkey;

    Center for Biophotonics Science and Technology, University of California Davis, Sacramento, CA, 95817, USA,Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, CA, 95817, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    SERS; plasmonic; protein; nanovoid; 3D nanostructures;

    机译：SERS;等离子体蛋白;纳米空隙3D纳米结构;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号