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Radiative Engineering of NanoAntenna Arrays for Ultra-Sensitive Vibrational Spectroscopy of Proteins

机译:蛋白质的超灵敏振动光谱的纳米天线阵列的辐射工程。

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Infrared absorption spectroscopy offers direct access to the vibrational signatures of molecular structure. Although absorption cross sections are nearly 10 orders of magnitude larger than the Raman cross sections, they are small in comparison with those of fluorescent labels. Sensitivity improvements are therefore required in order for the method to be applicable to single molecule/monolayer studies. In this work, we demonstrate a plasmon enhanced vibrational spectroscopy technique which allow for the measurement of molecule specific signatures at the monolayer level. Specifically, we show 4-5 order of magnitude enhancement of the amide-I and II backbone signature of protein monolayers, the signal resulting from only zeptomole quantities of molecules.
机译:红外吸收光谱法可直接访问分子结构的振动特征。尽管吸收截面比拉曼截面大了近10个数量级,但与荧光标记的吸收截面相比,它们却很小。因此,需要提高灵敏度才能使该方法适用于单分子/单分子层研究。在这项工作中,我们演示了一种等离激元增强的振动光谱技术,该技术可用于在单层水平上测量分子的特定标记。具体来说,我们显示了蛋白质单层酰胺-I和II主链签名的4-5个数量级的增强,该信号仅来自分子的分子数量。

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