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首页> 外文会议>Proceeding of International Apple Symposium 2002 null >Starch metabolism and subcellular localization of amylase in developing apple fruit
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Starch metabolism and subcellular localization of amylase in developing apple fruit

机译：苹果果实发育中淀粉的淀粉代谢和淀粉酶的亚细胞定位

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摘要

Starch degradation in cells is closely associated with cereal seed germination, photosynthesis in leaves, carbohydrate storage in tuberous roots and flesh fruit development. α- and β-amylase are considered the key enzymes catalyzing starch breakdown, but up to date their roles in starch breakdown in living cells remain unclear because the enzymes were shown often extrachloroplastic in living cells. The present experiment showed that α- and β-amylase activities were progressively increasing concomitantly with decreasing starch concentrations during apple fruit development. The enzyme subcellular localization studies via immunogold electron-microscopy technique showed that α- and β-amylase visualized by gold particles were both predominantly located in plastids, and the gold particles were scarcely found in other subcellular compartments. A high density of the enzyme was observed at periphery of starch granules during the middle and late developmental stages. These data proved that the enzymes are compartmented in their functional sites in living cells of the fruit. The predominantly plastid-distributed patterns of α- and β-amylases in cells was shown unchanged throughout the full stages of fruit development. So it is considered that α- and β-amylase are both involved in starch hydrolysis in plastids of the fruit cells.
机译：细胞中的淀粉降解与谷物种子发芽，叶片的光合作用，块根中的碳水化合物存储和果肉发育密切相关。 α-和β-淀粉酶被认为是催化淀粉分解的关键酶，但至今仍不清楚它们在活细胞中淀粉分解中的作用，因为这些酶经常在活细胞中显示出叶绿体增生作用。本实验表明，在苹果果实发育过程中，α-和β-淀粉酶活性随着淀粉浓度的降低而逐渐增加。通过免疫金电子显微镜技术进行的酶亚细胞定位研究表明，金颗粒可见的α-和β-淀粉酶均主要位于质体中，而在其他亚细胞区室中几乎找不到金颗粒。在发育的中后期，在淀粉颗粒的外围观察到高密度的酶。这些数据证明了酶在水果的活细胞中位于其功能部位。在整个果实发育的整个过程中，细胞中α-淀粉酶和β-淀粉酶的主要质体分布模式没有变化。因此认为α-和β-淀粉酶都参与了果实细胞质体中的淀粉水解。

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