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首页> 外文会议>Proceedings of 11th International Bhurban Conference on Applied Sciences amp; Technology >Prevalence and diagnosis of Hepatitis B virus based on Polymerase Chain Reaction (PCR) in district Noshki Balochistan
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Prevalence and diagnosis of Hepatitis B virus based on Polymerase Chain Reaction (PCR) in district Noshki Balochistan

机译：基于聚合酶链反应（PCR）的Noshki Balochistan地区乙型肝炎病毒的流行和诊断

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Hepatitis B virus (HBV) is major causative agent of chronic liver disease that leads to cirrhosis or hepatocellular carcinoma (HCC). It is estimated that about 5% patients became chronic carriers of this pathogen in Pakistan. HBV diagnosis is the most important for the patient treatment and vaccination. Serological tests are used to distinguish acute, self limited infections from chronic HBV infections and to monitor vaccine induced immunity. Nucleic acid testing for HBV-DNA by PCR is increasingly being used to quantify HBV viral load and measure the effectiveness of therapeutic agents. The aim of this study is to determine the prevalence of HBV in Noshki, Baluchistan. The study was conducted from September to December 2013 in Baluchistan University of Engineering Technology Information Management Sciences (BUETIMS) Quetta, Baluchistan. One hundred immunochromatography test (ICT) positive samples were collected for the analysis of HBV using nested PCR. Out of 100 subjects (64%) subjects were male and (36%) subjects were female having four different age groups between 15–60 years. A total of (4.0%) subjects were found positive for HBV DNA. Age, gender, literacy and marital status were determined. The prevalence of HBV infection was found more in age group 4 (51–60 years) than age group 1(15–30) and 3(41–50). There was no HBV DNA found in age group 2. The high prevalence HBV percentage was observed for males (4.78%) than females (2.69%). However, significantly increased association was found in illiterate population (6.0%) than literate population (2.0%). Higher prevalence rate of HBV was observed in married subjects (4.69%) than unmarried 2.78%). In conclusion the molecular diagnosis of HBV by Nested-PCR is more sensitive and specific for the determination HBV DNA. The prevalence and incidence of HBV in present study is alarming. The control and prevention of the local population from this fatal liver disease needs immediate attention.
机译：乙型肝炎病毒（HBV）是导致肝硬化或肝细胞癌（HCC）的慢性肝病的主要病因。据估计，巴基斯坦约有5％的患者成为该病原体的慢性携带者。 HBV诊断对患者的治疗和疫苗接种最为重要。血清学检测可用于区分急性，自我限制型感染和慢性HBV感染，并监测疫苗诱导的免疫力。通过PCR对HBV-DNA进行核酸检测的方法越来越多地用于量化HBV病毒载量和衡量治疗药物的有效性。这项研究的目的是确定Bal路支斯坦岛Noshki的HBV患病率。这项研究于2013年9月至2013年12月在Bal路支省uch路支工程技术信息大学（BUETIMS）进行。使用巢式PCR收集了一百份免疫色谱测试（ICT）阳性样品用于HBV分析。在100名受试者中（64％），男性为受试者，女性（36％）为15-60岁之间的四个年龄段的女性。总计（4.0％）的受试者被发现HBV DNA阳性。确定了年龄，性别，识字率和婚姻状况。在4岁组（51-60岁）中发现HBV感染的发生率高于1岁组（15-30）和3岁组（41-50）。在2岁年龄组中未发现HBV DNA。男性（4.78％）高于女性（2.69％）的HBV流行率。但是，文盲人口（6.0％）比识字人口（2.0％）的联想显着增加。已婚受试者中的HBV患病率较高（4.69％），未婚受试者中为2.78％）。总之，通过巢式PCR对HBV进行分子诊断对于测定HBV DNA更为灵敏和特异。目前研究中HBV的患病率和发病率令人震惊。必须立即注意控制和预防当地居民罹患这种致命性肝病。

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