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首页> 外文会议>Proceedings of the 13th IASTED international conference on intelligent systems and control; Proceedings of the Second IASTED international conference on Computational Bioscience. >MEASUREMENT AND MAPPING OF CELL TRACTION FORCES ON LIQUID CRYSTAL BASED FORCE TRANSDUCER
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MEASUREMENT AND MAPPING OF CELL TRACTION FORCES ON LIQUID CRYSTAL BASED FORCE TRANSDUCER

机译：基于液晶的力传感器的细胞牵引力的测量与映射

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A novel cell traction force transducer based on shearrnsensitive cholesteryl ester liquid crystals (LCTFT) hasrnbeen developed. This transducer incorporated with arncustom-built cell traction force measurement and mappingrn(CTFM) software showed improved sensitivity and spatialrnresolution in sensing traction forces of humanrnkeratinocytes. The sensing output of the LCTFT wasrndisplayed as linear deformation lines in the liquid crystalrnsurface that were induced by contraction of actinrnfilaments via the focal adhesions of cells. In this context,rnthe traction forces that were exerted locally by twornclusters of focal adhesions induced strains in bi-axialrndirections in the liquid crystal surface. By using cellrnrelaxation technique, these strains were determined andrnthey were correlated by Poisson’s ratio. With therndisplacement information, traction forces of single cellsrnwere calculated based on Hooke’s theorem and the forcedeformationrnrelationships were derived. In this study, therntransducer detected a wide range of cell traction forcesrn(～10 - 140 nN) indicating the sensitivity of the system.rnThe cell traction force maps generated by our CTFMrnsoftware at a spatial resolution of ～ 5μm showed goodrnrepresentations of localised cell traction force fields andrnmight be useful for time-based study of dynamic cellsrntractions
机译：已经开发了一种基于对剪切敏感的胆固醇酯液晶（LCTFT）的新型细胞牵引力传感器。该换能器与arncustom构建的细胞牵引力测量和制图软件（CTFM）结合使用，在感知人类角质形成细胞的牵引力方面显示出更高的灵敏度和空间分辨率。 LCTFT的感测输出在液晶表面上显示为线性变形线，该线性变形线是通过细胞的粘着斑由肌动蛋白丝的收缩引起的。在这种情况下，由粘着力的两个簇局部施加的牵引力在液晶表面的双轴方向上引起应变。通过细胞松弛技术，确定了这些菌株，并与泊松比相关。利用位移信息，根据胡克定理计算出单细胞的牵引力，得出了力-变形关系。在这项研究中，换能器检测到了广泛的细胞牵引力rn（〜10-140 nN），表明了系统的灵敏度。rn由我们的CTFM软件生成的细胞牵引力图在空间分辨率约为5μm时显示了良好的局部细胞牵引力表示。场和力对于基于时间的动态细胞牵引研究很有用

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