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首页> 外文会议>Proceedings of the 17th IASTED international conference on robotics applications ; Proceedings of the 11th IASTED international conference on biomedical engineering >MICROFLUIDIC DEVICES AS IN-VITRO MICROENVIRONMENTS FOR STEM CELL CULTURE
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MICROFLUIDIC DEVICES AS IN-VITRO MICROENVIRONMENTS FOR STEM CELL CULTURE

机译：微流控设备作为干细胞培养的体外微环境

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Many potential therapies are currently being studied that may promote neural regeneration and guide regenerating axons to form correct connections following injury. It has been shown that adult neurons have some limited regenerative capabilities, and the lack of connection formation between neurons is not an intrinsic inability of these cells to form axons after being damaged, but rather the inhibitory microenvironment of the injured tissue prevents regeneration. In this study, the polarization and chemotaxis of neuronal stem cells (NSC) in response to quantified gradients of nerve growth factor (NGF) was examined. To accomplish this, a microfluidic device was designed and fabricated to generate stable concentration gradients of biomolecules in a cell culture chamber within a 3D microenvironment. Numerical simulation was implemented to optimize the device geometry for generating a uniform concentration gradient of NGF which was found to remain stable for multiple hours. NSCs migration was studied within this microfluidic device in response to NGF concentration and within a 3D environment of collagen matrix. This device is expected to have wide applicability in the study of shear-sensitive cells such as NSC and non-adherent cell types as well as in the study of migration through three dimensional matrices.
机译：当前正在研究许多潜在的疗法，这些疗法可以促进神经再生并引导再生轴突在受伤后形成正确的连接。已经显示，成年神经元具有有限的再生能力，并且神经元之间缺乏连接形成不是这些细胞在受损后固有地无法形成轴突，而是受损伤组织的抑制性微环境阻止了再生。在这项研究中，神经干细胞（NSC）的极化和趋化响应神经生长因子（NGF）的定量梯度进行了检查。为实现此目的，设计并制造了一种微流体装置，以在3D微环境中的细胞培养室内生成稳定的生物分子浓度梯度。进行了数值模拟，以优化设备几何形状，以生成均匀的NGF浓度梯度，发现该NGF可以在多个小时内保持稳定。响应NGF浓度和胶原基质的3D环境，研究了该微流体装置中NSC的迁移。预期该装置在剪切敏感细胞（如NSC和非贴壁细胞类型）的研究以及通过三维矩阵迁移的研究中具有广泛的适用性。

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