Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa

机译：苜蓿液泡状Na + / H +反转运蛋白基因的克隆与表达分析

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A full-length cDNA, named MsNHXl, encoding vacuolar Na+/H+antiporter was cloned from alfalfa (Medicago sativa) , using degenerate primers, followed by 3 ' and 5 RACE. The cDNA sequence was 2225bp long and included an open reading frame encoding a deduced protein of 541 -amino -acid polypeptide. The deduced amino acid sequence showed high similarity (more than 73%) to those of the previously cloned Na+/H+antiporters form Arabidopsis thaliana, Qryza sativa, Atripkx gemlinin, Beta vulgaris and Hordeum vulgare. Southern hybridization showed that MsNHX has only one copy in alfalfa genome. Semiquantitative RT -PCR analysis indicates that the mRNA level of MsNHXl was moderate without stress and markedly up -regulated after treatment by NaCl and ABA (abscisic acid). Those results suggest that the MsNHXl product play an important role in salt tolerance of the alfalfa, and its transcript expression is possibly partially regulated through ABA -dependent signaling pathway.

机译：使用简并引物，随后是3'和5 RACE，从苜蓿（苜蓿）中克隆了编码液泡状Na + / H +反转运蛋白的全长cDNA，称为MsNHX1。 cDNA序列为2225bp长，并且包括编码541-氨基酸多肽的推导蛋白的开放阅读框。推导的氨基酸序列与拟南芥，Qryza sativa，Atripkx gemlinin，Beta vulgaris和大麦（Hordeum vulgare）先前克隆的Na + / H +反向转运蛋白具有高度相似性（超过73％）。 Southern杂交表明，MsNHX在苜蓿基因组中只有一个拷贝。半定量RT-PCR分析表明，MsNHX1的mRNA水平是中等的，没有压力，并且在用NaCl和ABA（脱落酸）处理后显着上调。这些结果表明，MsNHX1产物在紫花苜蓿的耐盐性中起重要作用，并且其转录物表达可能通过ABA依赖性信号传导途径被部分调节。

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来源
《Proceedings of 2010' International Symposium on forage, turf-grass and biofuel germplasm research.》|2010年|p.183-190|共8页
会议地点 Yangling(CN);Yangling(CN)
作者
QINGCHUAN YANG; MINGSHENG WU; PINGQING WANG; JUNMEI KANG; XINGLONG ZHOU;
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作者单位

Turf Forage Science,Institute of Animal Sciences,Chinese Academy of Agricultural Science (CAAS),Beijing,China,100094;

Bioengineering College,Chongqing Universtiy,Chongqing,China,400044 Beijing Seed Administration Station,Beijing,China,100044;

Bioengineering College,Chongqing Universtiy,Chongqing,China,400044;

Turf Forage Science,Institute of Animal Sciences,Chinese Academy of Agricultural Science (CAAS),Beijing,China,100094;

Bioengineering College,Chongqing Universtiy,Chongqing,China,400044;

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会议组织
原文格式 PDF
正文语种 eng
中图分类草地学、草原学;草地学、草原学;
关键词
Alfalfa; Na+/H+ antiporter; vacuole; RT-PCR;

机译：苜蓿; Na + / H +反向转运蛋白;真空; RT-PCR;

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专利

1. Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa [J] . Yang QC, Wu MS, Wang PQ, DNA Sequence: The Journal of DNA Sequencing and Mapping . 2005,第5期

机译：苜蓿液泡状Na + / H +反转运蛋白基因的克隆和表达分析
2. Effects of ABA, auxin, and gibberellin on the expression of genes for vacuolar H+ -inorganic pyrophosphatase, H+ -ATPase subunit A, and Na+/H+ antiporter in barley [J] . Fukuda A, Tanaka Y Plant Physiology and Biochemistry . 2006,第5a6期

机译：ABA，生长素和赤霉素对大麦液泡中H +-无机焦磷酸酶，H + -ATPase A亚基和Na + / H +反转运蛋白基因表达的影响
3. Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress [J] . Metwali Ehab M. R., Soliman Hemaid I. A., Fuller M. P., Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants . 2015,第2期

机译：盐胁迫下无花果液泡Na + / H +反转运蛋白基因（AgNHX1）的分子克隆和表达
4. Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa [C] . QINGCHUAN YANG, MINGSHENG WU, PINGQING WANG, International Symposium on forage, turf-grass and biofuel germplasm research . 2010

机译：Alfalfa的克隆和表达分析苜蓿β+ / H +抗抗原基因的克隆及表达分析
5. Vacuolar Na+/H+ Antiporters in Arabidopsis thaliana: Cation Selectivity and Biological Functions [D] . Zhang, Shiqi 2018

机译：拟南芥中的液泡Na + / H +反转运蛋白：阳离子选择性和生物学功能。
6. Cloning and Functional Characterization of a Vacuolar Na+/H+ Antiporter Gene from Mungbean (VrNHX1) and Its Ectopic Expression Enhanced Salt Tolerance in Arabidopsis thaliana [O] . Sagarika Mishra, Hemasundar Alavilli, Byeong-ha Lee, 2010

机译：拟南芥Vacular Na + / H +反转运蛋白基因（VrNHX1）的克隆，功能表征及其异位表达增强了拟南芥的耐盐性
7. Cloning and functional characterization of a vacuolar Na+/H+ antiporter gene from mungbean (VrNHX1) and its ectopic expression enhanced salt tolerance in Arabidopsis thaliana. [O] . Sagarika Mishra, Hemasundar Alavilli, Byeong-ha Lee, 2014

机译：绿豆液泡Na + / H +逆向转运蛋白基因的克隆和功能鉴定（VrNHX1）及其在拟南芥中的异位表达增强的耐盐性。

Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa

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