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首页> 外文会议>Proceedings of 4th international symposium on pesticides and environmental safety amp; 5th pan Pacific confernce on pesticide science amp; 8th international workshop on crop protection chemistry and regulatory harmonization >Comparative Transcriptional Profiling of Neurospora crassa and Fusarium graminearum in Response to Fungicides
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Comparative Transcriptional Profiling of Neurospora crassa and Fusarium graminearum in Response to Fungicides

机译：芥菜神经孢霉和禾谷镰刀菌对杀真菌剂的比较转录分析

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@@ Many site-specific fungicides have been developed to control plant diseases, and their precious antifungal mechanisms and target protein/enzymes have been determined in most of fungicides. However, effect of fungicides on genome-wide gene expression has not been fully understood. We used Neurospora crassa as a model fungus to identify a subset of genes differentially expressed in response to fungicides such as fludioxonil (stress response MAP kinase activator)，menadione (oxidizing agent), azoxystrobin (mitochondrial complex Ⅲ inhibitor), fluazinam (uncoupler), mepanipyrim (secretion inhibitor), fluconazole (Lanosterol 14-alphademethylase inhibitor), fenpropimorph (C-14 sterol reductase inhibitor), amphotericin B (sterol-binding agent), micafungin (beta-(1, 3)-glucan synthase inhibitor). To select the early response genes by target protein inhibition, growing hyphae were treated with fungicides for 60 min then RNAs were extracted. We selected the candidate genes using microarray analysis and identified the fungicideresponse genes using quantitative real-time PCR.
机译：已经开发了许多针对位点的杀真菌剂来控制植物病害，并且在大多数杀真菌剂中都已确定了它们的珍贵抗真菌机制和目标蛋白质/酶。但是，尚未完全了解杀真菌剂对全基因组基因表达的影响。我们以神经孢霉为模型真菌，鉴定了对杀真菌剂的响应差异表达的基因的子集，例如氟地西尼（应力响应MAP激酶激活剂），甲萘醌（氧化剂），嘧菌酯（线粒体复合物Ⅲ抑制剂），氟嗪南（解偶联剂）， mepanipyrim（分泌抑制剂），fluconazole（羊毛甾醇14-α-脱甲基酶抑制剂），fenpropimorph（C-14固醇还原酶抑制剂），两性霉素B（固醇结合剂），micafungin（β-（1，3）-葡聚糖合酶抑制剂）。为了通过靶蛋白抑制作用选择早期反应基因，将生长的菌丝用杀真菌剂处理60分钟，然后提取RNA。我们使用微阵列分析选择了候选基因，并使用定量实时PCR鉴定了杀菌剂响应基因。

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