Monoclonal Antibody Binding to (Fluoro) Quinolones in Competitive Immunoassay: Quantitive Correlation Antibody Affinity with Molecular Parameters

机译：竞争性免疫测定中的（氟）喹诺酮类单克隆抗体结合：分子参数的定量相关抗体亲和力。

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

@@ Antibodies used in competitive immunoassay tor the detection ot small molecule usually recognize one more molecules that share common epitopes with different affinities. For immunochemist, there is a challenging problem to well understand the cause of the antibody' s multi-specificity from only looking two-dimensional structural formulas of the compounds studied (Sanvicens et al.，2003). A method that can provide useful parameters about the topological and electrostatic properties of molecule can be sufficient in elucidating the diversity of antibody binding (Beier and Stanker 2001). In the study, we determinates the affinities of one monoclonal antibody (C4A9H1) to 14 (fluoro) quinolones by competitive ELISA and calculated molecular parameters of these (fluoro)quinolones by quantum chemical B3LYP/6-31G*.

机译：在竞争性免疫测定中用于检测小分子的抗体通常会识别另外一种分子，这些分子共享具有不同亲和力的共同表位。对于免疫化学家来说，仅从所研究化合物的二维结构式上看，就很难很好地理解抗体多特异性的原因（Sanvicens等，2003）。可以提供有关分子的拓扑和静电特性的有用参数的方法足以阐明抗体结合的多样性（Beier和Stanker 2001）。在这项研究中，我们通过竞争性ELISA确定了一种单克隆抗体（C4A9H1）与14种（氟）喹诺酮的亲和力，并通过量子化学B3LYP / 6-31G *计算了这些（氟）喹诺酮的分子参数。

著录项

来源
《Proceedings of 4th international symposium on pesticides and environmental safety amp; 5th pan Pacific confernce on pesticide science amp; 8th international workshop on crop protection chemistry and regulatory harmonization》|2012年|449-450|共2页
会议地点 Beijing(CN)
作者
Zhanhui Wang;
展开▼
作者单位

Department of Veterinary Pharmacology and Toxicology, college of Veterinary Medicine, China Agricultural University, Beijing 100193, China;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类植物化学保护理论;农用化学物质、有毒化学物质污染及其防治;
关键词
monoclonal antibody; (fluoro)quinolones; immunoassay; antibody affinity; molecular parameters;

机译：单克隆抗体;（氟）喹诺酮类;免疫测定;抗体亲和力;分子参数;

相似文献

外文文献
中文文献
专利

1. Development of a Generic Enzyme-Immunoassay for the Detection of Fluoro(quinolone)-Residues in Foodstuffs Based on a Highly Sensitive Monoclonal Antibody [J] . Acaroz Ulas, Dietrich Richard, Knauer Maria, Food analytical methods . 2020,第3期

机译：基于高敏感的单克隆抗体，在食品中检测氟（喹诺酮） - 氟（喹诺酮） - 份荧光酶 - 免疫测定的发展
2. Broad-Specificity Chemiluminescence Enzyme Immunoassay for (Fluoro)quinolones: Hapten Design and Molecular Modeling Study of Antibody Recognition [J] . Zeng Haopeng, Chen Jiahong, Zhang Chijian, Analytical chemistry . 2016,第7期

机译：（氟）喹诺酮类药物的广谱化学发光酶免疫分析：抗体识别的半抗原设计和分子建模研究
3. Binding properties of a monoclonal antibody against the Cry1Ab from Bacillus Thuringensis for the development of a capillary electrophoresis competitive immunoassay [J] . Cristina Giovannoli, Laura Anfossi, Claudio Baggiani, Analytical and Bioanalytical Chemistry . 2008,第3期

机译：苏云金芽孢杆菌Cry1Ab单克隆抗体的结合特性，用于开发毛细管电泳竞争性免疫分析
4. Monoclonal Antibody Binding to (Fluoro) Quinolones in Competitive Immunoassay: Quantitive Correlation Antibody Affinity with Molecular Parameters [C] . Zhanhui Wang International workshop on crop protection chemistry and regulatory harmonization . 2012

机译：在竞争性免疫测定中的单克隆抗体结合（氟）喹诺酮类：分子参数的定量相关抗体亲和力
5. PRODUCTION AND USE OF MONOCLONAL ANTIBODIES FOR MOLECULAR CHARACTERIZATION, AFFINITY PURIFICATION, AND SEROLOGICAL DETECTION OF ECONOMICALLY SIGNIFICANT PLANT VIRUSES [D] . DIACO, ROBERT 1985

机译：单克隆抗体的生产和用途，用于分子鉴定，亲和纯化和经济检测重要植物病毒的血清学检测
6. Physiologically relevant binding affinity quantification of monoclonal antibody PF‐00547659 to mucosal addressin cell adhesion molecule for in vitro in vivo correlation [O] . Mengmeng Wang, Amanda K Kussrow, Mireia Fernandez Ocana, 2017

机译：单克隆抗体PF-00547659与细胞黏附分子黏膜地址的生理相关结合亲和力定量用于体外体内相关性
7. Immunoassays for Affinity, Avidity and Competition: Methods to Evaluate Monoclonal and Polyclonal Antibodies Useful Assays in Development of New Antibodies, Better Understanding of Commercial Antibodies: Their Usefulness in Specific Aims of Research [O] . Anita Lewis Antes 2016

机译：用于亲和力，亲和力和竞争的免疫测定：评估单克隆和多克隆抗体的方法在新抗体的发育中有用的测定，更好地了解商业抗体：它们在具体目标的实用性
8. Detection of Rotavirus with a New Polyclonal Antibody Enzyme Immunoassay (Rotazyme 2) and a Commercial Latex Agglutination Text (Rotalex): Comparison with a Monoclonal Antibody Enzyme Immunoassay [R] . Doern, G. V. , Herrmann, J. E. , Henderson, P. , 1986

机译：用新的多克隆抗体酶免疫分析（Rotazyme 2）和商业乳胶凝集文本（Rotalex）检测轮状病毒：与单克隆抗体酶免疫分析比较

Monoclonal Antibody Binding to (Fluoro) Quinolones in Competitive Immunoassay: Quantitive Correlation Antibody Affinity with Molecular Parameters

摘要

著录项

相似文献

相关主题

期刊订阅