Unraveling the Role of Phe82 and Phe351 in Auxin-induced Substrate Perception by TIR1 Ubiquitin Ligase

机译：揭示Phe82和Phe351在TIR1泛素连接酶诱导的生长素诱导的底物感知中的作用

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

@@ It is well known that Auxin plays a key role in controlling many aspects of plant growth and development. Crystal structures of Transport inhibitor response 1 (TIR1), a true receptor of auxin, were very recently determined for TIR1 alone and in complexes with auxin and different synthetic analogues and an Auxin/Indole-3-Acetic Acid (Aux/IAA) substrate peptide. However, the dynamic conformational changes of the key residues of TIR1 that take place during the auxin and substrate perception by TIR1 and the detailed mechanism of these changes are still unclear. In the present study, various computational techniques were integrated to uncover the detailed molecular mechanism of the auxin and Aux/IAA perception process; these simulations included molecular dynamics (MD) simulations on complexes and the free enzyme, the molecular mechanics Poisson Boltzmann surface area (MM-PBSA) calculations, normal mode analysis, and hydrogen bond energy (HBE) calculations.

机译：@@众所周知，生长素在控制植物生长和发育的许多方面起着关键作用。最近确定了单独的TIR1以及与生长素和不同的合成类似物以及Auxin / Indole-3-乙酸（Aux / IAA）底物肽复合的TIR1的运输抑制剂反应1（TIR1）的晶体结构，它是生长素的真正受体。。然而，尚不清楚在生长素和底物被TIR1感知期间TIR1关键残基的动态构象变化以及这些变化的详细机理。在本研究中，整合了各种计算技术以揭示生长素和Aux / IAA感知过程的详细分子机制；这些模拟包括对复合物和游离酶的分子动力学（MD）模拟，泊松玻尔兹曼表面积（MM-PBSA）分子力学计算，正态分析和氢键能（HBE）计算。

著录项

来源
《Proceedings of 4th international symposium on pesticides and environmental safety amp; 5th pan Pacific confernce on pesticide science amp; 8th international workshop on crop protection chemistry and regulatory harmonization》|2012年|454|共1页
会议地点 Beijing(CN)
作者
Gefei Hao; Guangfu Yang;
展开▼
作者单位

Key Laboratory of Pesticide Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China;

Key Laboratory of Pesticide Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类植物化学保护理论;农用化学物质、有毒化学物质污染及其防治;
关键词
auxin; molecular dynamics; TIR1 Ubiquitin Ligase;

机译：生长素；分子动力学； TIR1泛素连接酶;

相似文献

外文文献
中文文献
专利

1. Mechanism of auxin perception by the TIR1 ubiquitin ligase [J] . Xu Tan, Luz Irina A. Calderon-Villalobos, Michal Sharon, Nature . 2007,第7136期

机译：TIR1泛素连接酶对生长素的感知机制
2. KCTD10 Biology: An Adaptor for the Ubiquitin E3 Complex Meets Multiple Substrates Emerging Divergent Roles of the cullin-3/KCTD10 E3 Ubiquitin Ligase Complex in Various Cell Lines [J] . Maekawa Masashi, Higashiyama Shigeki BioEssays : . 2020,第8期

机译：KCTD10生物学：泛素E3复合物的适配器符合各种细胞系中核蛋白-3 / KCTD10 E3泛素连接酶复合物的多个底物出现的多种底物
3. The G1 phase E3 ubiquitin ligase TRUSS that gets deregulated in human cancers is a novel substrate of the S-phase E3 ubiquitin ligase Skp2 [J] . Jamal Azfar, Swarnalatha Manickavinayaham, Sultana Sarwat, Cell cycle . 2015,第16期

机译：在人类癌症中被放松调节的G1期E3泛素连接酶TRUSS是S期E3泛素连接酶Skp2的新型底物
4. Unraveling the Role of Phe82 and Phe351 in Auxin-induced Substrate Perception by TIR1 Ubiquitin Ligase [C] . Gefei Hao, Guangfu Yang International workshop on crop protection chemistry and regulatory harmonization . 2012

机译：通过TiR1泛素连接酶解开PHE82和PHE351在肿瘤诱导的基质感知中的作用
5. Using Proximity-based Proteomics to Identify Interacting Partners and Substrates for the Listerin Ubiquitin Ligase [D] . Zuzow, Nathaniel David. 2016

机译：使用基于接近的蛋白质组学来识别汉替林蛋白质酶的互动伴侣和底物
6. The Role of Phe82 and Phe351 in Auxin-Induced Substrate Perception by TIR1 Ubiquitin Ligase: A Novel Insight from Molecular Dynamics Simulations [O] . Ge-Fei Hao, Guang-Fu Yang 2010

机译：Phe82和Phe351在TIR1泛素连接酶诱导的生长素诱导的底物感知中的作用：分子动力学模拟的新见解。
7. The Role of Phe82 and Phe351 in Auxin-Induced Substrate Perception by TIR1 Ubiquitin Ligase: A Novel Insight from Molecular Dynamics Simulations [O] . Hao, Ge-Fei, Yang, Guang-Fu 2010

机译：Phe82和Phe351在TIR1泛素连接酶诱导的生长素诱导的底物感知中的作用：分子动力学模拟的新见解。

Unraveling the Role of Phe82 and Phe351 in Auxin-induced Substrate Perception by TIR1 Ubiquitin Ligase

摘要

著录项

相似文献

相关主题

期刊订阅