• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Proceedings of the international conference on germplasm of ornamentals >Genetic Diversity of Wild Daylily in Taihang Mountain Areas Based on ISSR Markers
【24h】

Genetic Diversity of Wild Daylily in Taihang Mountain Areas Based on ISSR Markers

机译：基于ISSR标记的太行山地区野生黄花菜遗传多样性

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Daylily (Hemerocallis spp.) is widely cultivated due to its showy flowers and its adaptation to a wide range of soil and climates.There are 18 species in Hemerocallis, of which 11 are native to China.Daylily is one of the extensively bred plant genera and the cultivars are highly diverse in flower colour and form.Understanding the genetic diversity of wild germplasm can help breeders further improve daylily cultivars.Twenty-one accessions of Hemerocallis spp.were collected from ten counties along the Taihang Mountain range.The plants were evaluated at the field station of the Institute of Horticulture, Shanxi Academy of Agricultural Sciences.Significant morphological differences were observed among the accessions.Seven accessions collected were triploid and all the rest were diploid.Genetic variation within the wild collection and between the wild collection and 16 commercial cultivars (ten from USA, five from New Zealand and one from China) were analysed using six Inter-Simple Sequence Repeat (ISSR) primers.A total of 120 polymorphic bands were obtained.Genetic diversity was high among the collected genotypes with 95％ polymorphic loci (PPL), but was relatively low within commercial cultivars (PPL=89％).The genetic diversity among commercial cultivars within each country were low (PPL=72％ for USA and 51％ for New Zealand).A dendrogram of the 37 genotypes were constructed using BioNJ method.Pairwise similarity coefficients among the accessions ranged from 0.14 to 0.73.The genotypes were classified into three groups based on our results.All wild collections and one Chinese cultivar 'daylily' were grouped in one cluster.The cultivars from USA and New Zealand were distributed into two other clusters.The wild collections were divided into two subgroups.However, the subgroups do not reflect geographic locations of the wild genotypes.These results demonstrated that ISSR profiling is a powerful method for the identification of genetic variation of daylily cultivars and wild collections.However, a larger number and higher density of natural accessions should be collected and analysed, and more primers need to be employed for characterisation of wild collections at population level.
机译：黄花菜（Hemerocallis spp。）因其艳丽的花朵以及对多种土壤和气候的适应性而得到广泛栽培。萱草有18种，其中11种是中国本土的。黄花菜是广泛繁殖的植物属之一了解野生种质的遗传多样性可以帮助育种者进一步改善黄花菜品种。从太行山沿岸十个县收集二十一个萱草属植物，并对植物进行评价。在山西省农业科学院园艺研究所田间站观察到的种质之间存在显着的形态学差异，收集到的7个种为三倍体，其余的均为二倍体。野生种内以及野生种与16种之间的遗传变异使用六个Inter-Simple Sequenc对商业品种（来自美国的十个，来自新西兰的五个和来自中国的一个）进行了分析e重复（ISSR）引物，共获得120条多态性条带，遗传多样性在95％多态性位点（PPL）的基因型中较高，但在商业品种中相对较低（PPL = 89％）。各国商业品种之间的亲缘关系很低（美国为72％，新西兰为51％）。使用BioNJ方法构建了37个基因型的树状图，种质之间的成对相似系数在0.14至0.73之间。根据我们的结果将其分为三类，将所有野生品种和一个中国黄花菜``黄花菜''分组为一个类群，将来自美国和新西兰的品种分为两个其他类群，将野生类分为两个亚类。这些结果表明，ISSR谱分析是鉴定黄花菜和野生玉米遗传变异的有力方法。但是，应该收集和分析更多数量和更高密度的天然种质，并且需要使用更多的引物来表征种群水平的野生种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号