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首页> 外文会议>Proceedings of the international conference on germplasm of ornamentals >Cloning and Expression Analysis of a Novel Male Fertility-Related Gene in Petunia hybrida
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Cloning and Expression Analysis of a Novel Male Fertility-Related Gene in Petunia hybrida

机译：矮牵牛中一个新的雄性育性相关基因的克隆与表达分析

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摘要

A novel male fertility-related gene, Phmf2, was identified in SSH libraries constructed from abortive and normal flower buds of Petunia hybrida.Phmf2 was up-regulated in abortive flower buds.The full-length cDNA of Phmf2 was obtained by PCR amplification using gene-specific primers.It contains a 1,005-bp open reading frame, encoding 334 amino acids polypeptide.Predicted molecular weight is 37241.7 Da and estimated isoelectric ooint is 6.37.Seauence anaivsis showed that Phmf2 shared the highest similarity (91％) in amino acid sequence with oreviously recorded TKPR1 gene in Nicotiana tabacum and had the same high homologv (72％)with DRL1 gene in Medicago (MtDFR) and Arabidopsis (AtDRL1), all of which are essential for male fertility.Expression analysis by semi-quantitative RT-PCR showed that this gene highly expressed in abortive flower buds with none or little detected in normal flower buds or other organs.We thus predicted that Phmf2 should be male fertility-related and hope it could be used to create a male sterile line of Petunia hybrida or other ornamentals, so as to play a significant role in ornamentals breeding.
机译：在矮牵牛的流产和正常花芽构建的SSH文库中鉴定了一个新的雄性育性相关基因Phmf2，在流产花芽中上调了Phmf2，利用该基因通过PCR扩增获得了Phmf2的全长cDNA。特异性引物，包含一个1,005bp的开放阅读框，编码334个氨基酸的多肽，预计分子量为37241.7 Da，估计等电点为6.37。从基因分析来看，Phmf2在氨基酸序列中具有最高的相似性（91％）。烟草中以前记录的TKPR1基因，与紫花苜蓿（MtDFR）和拟南芥（AtDRL1）中的DRL1基因具有相同的高同源性（72％），它们都是雄性育性所必需的。用半定量RT-PCR分析表达表明该基因在流产花蕾中高表达，在正常花蕾或其他器官中几乎没有检测到，因此我们预测Phmf2应该与雄性育性有关，并希望它可能是用于创建矮牵牛或其他观赏植物的雄性不育系，从而在观赏植物育种中发挥重要作用。

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  • 来源
  • 会议地点 Beijing(CN)
  • 作者

    Y.Z.Yue; F.F.Ma; X.Huang; G.F.Liu; M.Z.Bao; H.R.Hua;

  • 作者单位

    Key Laboratory of Horticultural Plant Biology, Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 China;

    Key Laboratory of Horticultural Plant Biology, Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 China;

    Key Laboratory of Horticultural Plant Biology, Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 China;

    Key Laboratory of Horticultural Plant Biology, Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 China;

    Key Laboratory of Horticultural Plant Biology, Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 China;

    Key Laboratory of Horticultural Plant Biology, Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 观赏园艺（花卉和观赏树木）;
  • 关键词

    Phmf2; suppression subtractive hybridization; open reading frame; TKPRI; DRLI; semi-quantitative RT-PCR;

    机译：Phmf2；抑制消减杂交；开放阅读框； TKPRI； DRLI；半定量RT-PCR;

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