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首页> 外文会议>Proceedings of the international conference on germplasm of ornamentals >Cloning and Bioinformatics Analysis of ERF Response Factor Gene from Prunus mume
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Cloning and Bioinformatics Analysis of ERF Response Factor Gene from Prunus mume

机译：梅花ERF应答因子基因的克隆及生物信息学分析

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摘要

Primers were designed according to the sequence of the ERF (ethylene responsive factor) genes from GenBank.A fragment of the ERF response factor gene was isolated from Prunus mume by PCR and RT-PCR.The sequencing results showed that the sequences obtained by two approaches were the same.The full-length DNA was 816 bp long, and encoded a putative protein of 271 amino acids.On this basis, we analysed the structure and function of the sequence via bioinformation analysis using protocols and related software.Sequences analysis showed that the gene owns the characteristics of the ERF response factor, which contains an AP2 /EREB DNA-binding domain and two special short amino acids sequences.Homology analysis shows that the nucleotide were highly homologous to that of other species, especially the Malus×domestica.So we can predict that we have successfully cloned the ERF gene of Prunus mume.It will contribute to the follow-up downstream analysis and molecular breeding.
机译：根据GenBank中ERF（乙烯反应因子）基因的序列设计引物，通过PCR和RT-PCR从梅花中分离到ERF反应因子基因的片段。全长DNA全长816 bp，编码一个271个氨基酸的推定蛋白质。在此基础上，我们使用协议和相关软件通过生物信息分析对序列的结构和功能进行了分析。该基因具有ERP反应因子的特征，其具有AP2 / EREB DNA结合结构域和两个特殊的短氨基酸序列。同源性分析表明该核苷酸与其他物种，特别是Malus×domestica具有高度同源性。因此，我们可以预测我们已经成功克隆了梅花的ERF基因，这将有助于后续的下游分析和分子育种。

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