In situ PCR and immunohistochemical studies on p16 gene in pituitary adenomas

机译：垂体腺瘤中p16基因的原位PCR和免疫组化研究

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The genetic alteration of p16, a member of cyclin-dependent kinase inhibitor family, has been intensively investigated using the approaches of molecular biology. The homozygous deletion of p16 was reported in a large number of tumor cell lines. However, when a homogenate of a primary tumor was used for gene-deletion detection, non-neoplastic tissue admixed in specimens could contaminate the tumor DNA. In situ PCR allows a specific DNA fragment amplified within the original cell, then detected in situ. Since the cells are morphologically identified, one could know if a specific gene exists in a certain population of cells by in situ PCR techniques.

机译：p16是细胞周期蛋白依赖性激酶抑制剂家族的成员，其遗传改变已使用分子生物学方法进行了深入研究。在大量肿瘤细胞系中报道了p16的纯合缺失。但是，当使用原发肿瘤的匀浆进行基因缺失检测时，混入标本中的非肿瘤组织会污染肿瘤DNA。原位PCR可以在原始细胞中扩增特定的DNA片段，然后原位检测。由于细胞是通过形态学鉴定的，因此可以通过原位PCR技术知道特定基因是否存在于特定的细胞群中。

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《Proceedings of International Kunming Symposium on Microscopy July 2-5, 2000 Kunming, Yunnan Province, China》|2000年|p.183-184|共2页
会议地点 Kunming(CN);Kunming(CN)
作者
Jing Yi; Yuying Chen; Wenhao Xiong; Xiaoxiong Li; Xueming Tang;
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正文语种 eng
中图分类晶体学;
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1. Immunohistochemical Expression of p16 and p21 in Pituitary Tissue Adjacent to Pituitary Adenoma versus Pituitary Tissue Obtained at Autopsy: Is There a Difference? [J] . Gacic Emilija Manojlovic, Skender-Gazibara Milica, Soldatovic Ivan, Endocrine pathology . 2015,第2期

机译：p16和p21在垂体腺瘤附近的垂体组织中的免疫组织化学表达与在尸体解剖中获得的垂体组织的免疫组织化学表达：有区别吗？
2. Gene expression study of thyrotropin releasing hormone (TRH) receptor using RT-PCR: relationship to clinical and immunohistochemical phenotypes in a series of human pituitary adenomas. [J] . Igarashi Migitaka J, Yamada S, Hara M, Endocrine journal . 2003,第4期

机译：促甲状腺激素释放激素（TRH）受体的RT-PCR基因表达研究：与一系列人类垂体腺瘤的临床和免疫组化表型的关系。
3. Gene Expression Study of Thyrotropin Releasing Hormone (TRH) Receptor Using RT-PCR: Relationship to Clinical and Immunohistochemical Phenotypes in a Series of Human Pituitary Adenomas [J] . Junko IGARASHI-MIGITAKA, Kazuaki HIRATA, Masayuki HARA, Endocrine journal . 2003,第4期

机译：逆转录-聚合酶链反应（RT-PCR）促甲状腺激素释放激素（TRH）受体的基因表达研究：与一系列人类垂体腺瘤的临床和免疫组化表型的关系。
4. In situ PCR and immunohistochemical studies on p16 gene in pituitary adenomas [C] . Jing Yi, Yuying Chen, Wenhao Xiong, International Kunming symposium on microscopy . 2000

机译：原位PCR和垂体腺瘤P16基因的免疫组化研究
5. Pituitary gene expression in mice with altered growth hormone levels: Quantitative RT-PCR of transcription factors Pit -1, Zn -16, TEF, and Prop -1 [D] . Wojtkiewicz, Patrick Wray 1999

机译：改变生长激素水平的小鼠垂体基因表达：转录因子Pit -1，Zn -16，TEF和Prop -1的定量RT-PCR
6. Presence of neurophysins in the human pituitary corticotrophs Cushings adenomas and growth hormone-producing adenomas detected by immunohistochemical study. [O] . N. Kimura, N. Andoh, N. Sasano, 1986

机译：通过免疫组织化学研究检测到人垂体皮质激素库欣腺瘤和生长激素生成腺瘤中存在神经元。
7. Gene Expression Study of Thyrotropin Releasing Hormone (TRH) Receptor Using RT-PCR: Relationship to Clinical and Immunohistochemical Phenotypes in a Series of Human Pituitary Adenomas [O] . Junko IGARASHI-MIGITAKA, Shozo YAMADA, Masayuki HARA, 2003

机译：使用RT-PCR释放激素（TRH）受体的基因表达研究：一系列人脑腺癌中的临床和免疫组织化学表型

In situ PCR and immunohistochemical studies on p16 gene in pituitary adenomas

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