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首页> 外文会议>Proceedings of Life in space for life on earth >THE SIMBOX EXPERIMENT WITH ARABIDOPSIS THALIANA CELL CULTURES: HARDWARE-TESTS AND FIRST RESULTS FROM THE GERMAN-CHINESE SATELLITE MISSION SHENZHOU 8
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THE SIMBOX EXPERIMENT WITH ARABIDOPSIS THALIANA CELL CULTURES: HARDWARE-TESTS AND FIRST RESULTS FROM THE GERMAN-CHINESE SATELLITE MISSION SHENZHOU 8

机译：具有阿拉伯拟南芥细胞培养功能的SIMBOX实验：硬件测试和德国-中国卫星飞行任务的首次结果神舟8

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摘要

The Simbox experiment was the first joint German-rnChinese space project. In this context Arabidopsisrnthaliana cell cultures were exposed to microgravity forrna 17-day period. To carry out a successful spacernmission, diverse hardware tests were performed inrnadvance. Due to the limited oxygen supply inside thernhardware units, cells were fixed after 5 days underrnmicrogravity conditions. As a control, samples werernexposed in an on-board 1g reference centrifuge. Torninvestigate the space effect, a ground-based study wasrnperformed with the same hardware and identicalrnexperimental procedures. As we were able to obtainrnhigh quality RNA from the RNAlater quenchedrnsamples, we used the Affymetrix Arabidopsis genomernarray for a transcriptome analysis. Our experimentrnaimed at the identification of plant genes that wererndifferentially expressed after long-term exposure tornmicrogravity. Pair-wise comparison of flight samplesrnwith 1g controls revealed the largest differencesrnbetween space 1g and ground 1g controls.
机译：Simbox实验是德国与中国合作的第一个太空项目。在这种情况下，将拟南芥细胞培养物暴露于微重力法前17天。为了成功进行间隔发射，需要进行各种硬件测试。由于硬件单元内部的氧气供应有限，因此在微重力条件下放置5天后即可固定细胞。作为对照，将样品在车载1g参考离心机中暴露。在研究空间效应的过程中，以相同的硬件和相同的实验程序对地面进行了研究。由于我们能够从RNAlater淬灭的样品中获得高质量的RNA，因此我们将拟南芥Affymetrix基因组芯片用于转录组分析。我们的实验旨在鉴定长期暴露于微重力下差异表达的植物基因。飞行样本与1g控件的成对比较显示，空间1g和地面1g控件之间的最大差异。

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  • 会议地点 Aberdeen(GB)
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    Svenja Fengler; Maren Neef; Margret Ecke; Ruediger Hampp;

  • 作者单位

    University of Tuebingen, Physiological Ecology of Plants, Auf der Morgenstelle 1, 72076 Tuebingen (Germany) Email: Svenja.Fengler@uni-tuebingen.de;

    University of Tuebingen, Physiological Ecology of Plants, Auf der Morgenstelle 1, 72076 Tuebingen (Germany);

    University of Tuebingen, Physiological Ecology of Plants, Auf der Morgenstelle 1, 72076 Tuebingen (Germany);

    University of Tuebingen, Physiological Ecology of Plants, Auf der Morgenstelle 1, 72076 Tuebingen (Germany);

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