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首页> 外文会议>Proceedings vol.2004-12; Electrochemical Society(ECS) Annual Spring Meeting and International Symposium on Fullerenes, Nanotubes, and Carbon Nanoclusters; 20040509-13; San Antonio,TX(US) >SURFACE IMMOBILIZATION AND CHARACTERIZATION OF CYTOCHROME c ON FULLERENE MODIFIED ELECTRODES
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SURFACE IMMOBILIZATION AND CHARACTERIZATION OF CYTOCHROME c ON FULLERENE MODIFIED ELECTRODES

机译：富勒烯修饰电极上细胞色素c的表面固定化和鉴定

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摘要

Drop-coated fullerene film and fullerene-palladium (C_(60)-Pd) polymer film modified platinum, glassy carbon and indium-tin-oxide (ITO) electrodes were prepared and used for surface immobilization and electrochemical property investigation of bovine cytochrome c (cyt c). The immobilized cyt c was characterized by piezoelectric microgravimetry at a quartz crystal microbalance (QCM), UV-visible absorption and X-ray photoelectron spectroscopy (XPS), as well as cyclic voltammetry (CV) techniques. The UV-visible spectral studies revealed a small blue shift of both the Soret and Q band of the heme moiety of cyt c, immobilized on the C_(60)-Pd polymer film modified ITO electrode, as compared to the bands of cyt c in solution suggesting that molecules of cyt c are densely packed onto the surface of the modified electrode. The CV studies revealed a quasi-reversible electrode behavior of the heme moiety indicating the occurrence of kinetically hindered electron transfer. A good agreement was found between the values of cyt c electrode surface coverage determined by piezoelectric microgravimetry and cyclic voltammetry. The possible mechanisms of cyt c immobilization on the C_(60) film or C_(60)-Pd film modified electrodes are also discussed.
机译：制备了滴涂富勒烯膜和富勒烯-钯（C_（60）-Pd）聚合物膜修饰的铂，玻璃碳和氧化铟锡（ITO）电极，并将其用于牛细胞色素c的表面固定和电化学性质研究（ cyt c）。固定化的cyt c的特征是通过石英微天平（QCM）的压电微重力法，紫外可见吸收和X射线光电子能谱（XPS）以及循环伏安法（CV）进行的。紫外可见光谱研究表明，与Cy（c）（60）-Pd聚合物薄膜修饰的ITO电极相比，固定在C_（60）-Pd聚合物膜修饰的ITO电极上的Cyt c血红素部分的Soret和Q带都有小的蓝移。解决方案表明，cyt c分子紧密堆积在修饰电极的表面上。 CV研究揭示了血红素部分的准可逆电极行为，表明发生了动力学受阻的电子转移。在通过压电微重力法和循环伏安法测定的cyt c电极表面覆盖率值之间找到了很好的一致性。还讨论了将Cyt c固定在C_（60）膜或C_（60）-Pd膜修饰电极上的可能机制。

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