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首页> 外文会议>Progress in functional amino acids and carbohydrates for animal production >High level fusion expression of bovine lactoferricin and lactoferrampin gene in photorhabdus luminescens
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High level fusion expression of bovine lactoferricin and lactoferrampin gene in photorhabdus luminescens

机译：牛乳铁蛋白和乳铁蛋白基因在光致发光菌中的高水平融合表达

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摘要

The aims of this study were to produce a new antimicrobial peptide, bovine lactoferricin- lactoferrampin (LFC-LFA) in Photorhabdus luminescens (P. luminescens). cipA and cipB genes which encode crystalline inclusion proteins in a P. luminescens strain were deleted by using recombineering technology. The mutant of P. luminescens which defined as P. luminescens TZR001 was used as protein expression host. Bovine lactoferricin (LFC) and lactoferrampin (LFA) are two active fragments and are located in the N1-domain of bovine lactoferrin. Synthetic LFC-LFA gene containing LFC and LFA was fused with cipB gene and cloned into arabinose inducible expression vector to form the expression plasmid pBAD-cipB-LFC-LFA. After transforming the expression plasmid into host bacterial strain P. luminescens TZR001, cipB-LFC-LFA fusion protein was expressed when induced by L-arabinose and the amount of which reached to about 20% of the total bacterial protein. Experiments in vitro showed that LFC-LFA released from cipB had an antimicrobial activity.
机译：这项研究的目的是在光致发光杆菌（P. luminescens）中产生一种新的抗菌肽，牛乳铁蛋白-乳铁蛋白（LFC-LFA）。通过使用重组技术，删除了编码光致假单胞菌菌株中的晶体包涵蛋白的cipA和cipB基因。定义为发光假单胞菌TZR001的发光假单胞菌的突变体用作蛋白质表达宿主。牛乳铁蛋白（LFC）和乳铁蛋白（LFA）是两个活性片段，位于牛乳铁蛋白的N1结构域中。将包含LFC和LFA的合成LFC-LFA基因与cipB基因融合，并克隆到阿拉伯糖诱导型表达载体中，以形成表达质粒pBAD-cipB-LFC-LFA。在将表达质粒转化成宿主细菌菌株Luminescens TZR001之后，当被L-阿拉伯糖诱导时，表达了cipB-LFC-LFA融合蛋白，其量达到了总细菌蛋白的约20％。体外实验表明，从cipB释放的LFC-LFA具有抗菌活性。

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  • 来源
  • 会议地点 Guangzhou(CN)
  • 作者

    Z.R.Tang; Y.M.Zhang; Z.H.Sun; S.A.Francis; Jun Fu; M.M.Geng; Y.L.Yin;

  • 作者单位

    Laboratory for Agro-ecological Processes in Subtropical Region, Institute ofSubtropical Agriculture, CAS, Hunan 410125, China;

    Laboratory for Agro-ecological Processes in Subtropical Region, Institute ofSubtropical Agriculture, CAS, Hunan 410125, China Gene Bridges GmbH, BioInnovation Zentrum, Am Tatzberg 47, 01307, Dresden,Germany;

    Laboratory for Agro-ecological Processes in Subtropical Region, Institute ofSubtropical Agriculture, CAS, Hunan 410125, China;

    BioInnovation Zentrum, Technical University of Dresden, Am Tatzberg 47, 01307,Dresden, Germany;

    BioInnovation Zentrum, Technical University of Dresden, Am Tatzberg 47, 01307,Dresden, Germany;

    Laboratory for Agro-ecological Processes in Subtropical Region, Institute ofSubtropical Agriculture, CAS, Hunan 410125, China;

    Correspondence: Institute;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 饲料;
  • 关键词

    Bovine lactoferricin; Bovine lactoferampin; Photorhabdus luminescens TZR001; Recombineering; Antimicrobial peptides;

    机译：牛乳铁蛋白；牛乳铁蛋白发光Photorhabdus luminescens TZR001;重组；抗菌肽;

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