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Multimarker Approach Analysis in Clam Ruditapes decussatus Sampled from Industrial Area of Tunis Lake

机译:突尼斯湖工业区蛤Ru的多标记方法分析

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The purpose of this study is to assess the efficiency of a multimarker approach in clam Ruditapes decussatus sampled from industrial area of Tunis Lake. Clam samples were collected from industrial area through four seasons. Control samples were collected at the reference station "Ellouza". Benzo[a]pyrene hydroxylase (BPH) and Glutathione-S-transferase (GST) activities were used as a phase I and phase II detoxification enzyme. Catalase (CAT) activity and lipid peroxidation were analyzed as oxidative stress markers. Lipid peroxidation was assessed using malondialdehyde (MDA) accumulation. Results showed a significant increase of all biomarker levels compared with controls. Moreover, the responses of four biomarkers fluctuated over time. BPH and GST activities showed a progressive increase from winter to autumn. CAT activity reached a maximum value in spring; while MDA concentration recorded a maximum value in summer. Seasonal variation of biomarkers arises from a complex interaction between exogenous and endogenous factors. These results confirm the efficiency of the multimarker approach used in this study for the detection of biologic responses in Ruditapes decussatus to environmental pollution and to confirm the presence of anthropogenic contaminants in the area of study, which essentially due to the industrial rejections.
机译:这项研究的目的是评估从突尼斯湖工业区采样的蛤仔Ruditapes decussatus中多标记方法的效率。通过四个季节从工业区收集蛤样品。在参考站“ Ellouza”收集对照样品。苯并[a] py羟化酶(BPH)和谷胱甘肽-S-转移酶(GST)活性用作I期和II期解毒酶。过氧化氢酶(CAT)活性和脂质过氧化被分析为氧化应激指标。使用丙二醛(MDA)积累评估脂质过氧化。结果显示,与对照相比,所有生物标志物水平均显着增加。此外,四种生物标志物的反应随时间波动。从冬季到秋季,BPH和GST的活动显示出逐渐增加的趋势。春季的CAT活性达到最大值。夏季MDA浓度最高。生物标志物的季节性变化源于外源性和内源性因素之间的复杂相互作用。这些结果证实了本研究中使用的多标记方法的效率,该方法可用于检测硬皮芸苔对环境污染的生物学响应,并确认研究区域内存在人为污染物,这主要是由于工业废弃物。

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