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首页> 外文会议>Progress on post-genome technologies and modern natural products >CONSTRUCTION OF MIR-145 EUKARYOTIC EXPRESSION VECTOR AND ITS STABLE EXPRESSION IN HUMAN COLON CANCER CELLS
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CONSTRUCTION OF MIR-145 EUKARYOTIC EXPRESSION VECTOR AND ITS STABLE EXPRESSION IN HUMAN COLON CANCER CELLS

机译：MIR-145真核表达载体的构建及其在人结肠癌细胞中的稳定表达

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摘要

Recent research indicates that miR-145 is involved in various biological processes including cell cycle, cell differentiation, apoptosis and colorectal cancer. In this study, we report the construction of recombinant plasmid miR-145 expression vector and its stable expression of miR-145 in transfected human colon cancer cells (HCT-116). The double strands oligo encoding the miR-145 was designed and synthesized to generate the mature miR-145 expression plasmid. The new expression vector of miR-145 was named pHsa-miR-145. PHsa-miR-145 and its controls were transfected to HCT-116 cells. Fluorescence detection displayed that fluorescence intensity of GFP was highest during 48 and 72 hours posttransfection, and qRT-PCR showed a significantly increase in miR-145 expression in the vector transfected cells compared with the expression in its controls. The recombinant plasmid expression vector of miR-145 was constructed successfully, which may facilitate further study of its function in the process of cell cycle and colorectal cancer.
机译：最近的研究表明，miR-145参与了多种生物学过程，包括细胞周期，细胞分化，细胞凋亡和结肠直肠癌。在这项研究中，我们报道了重组质粒miR-145表达载体的构建及其在转染的人结肠癌细胞（HCT-116）中的稳定表达。设计并合成编码miR-145的双链寡核苷酸以产生成熟的miR-145表达质粒。 miR-145的新表达载体命名为pHsa-miR-145。将PHsa-miR-145及其对照转染至HCT-116细胞。荧光检测显示，GFP的荧光强度在转染后48和72小时内最高，并且qRT-PCR显示，与载体对照相比，载体转染细胞中miR-145表达显着增加。成功构建了miR-145的重组质粒表达载体，可能有助于进一步研究其在细胞周期和结直肠癌过程中的功能。

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  • 来源
  • 会议地点 Chongqing(CN)
  • 作者

    Liang Gaofeng; Hu Feihu; Chen Shanshan; Shao Youhua; Xiao Zhongdang;

  • 作者单位

    School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics,Southeast University, Nanjing, China;

    School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics,Southeast University, Nanjing, China;

    School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics,Southeast University, Nanjing, China;

    School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics,Southeast University, Nanjing, China;

    School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics,Southeast University, Nanjing, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物工程学（生物技术）;
  • 关键词

    miR-145; HCT-116; vector;

    机译：miR-145； HCT-116；向量;

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