PREPARATION OF DNA-FUNCTIONALIZED Pd NANOPARTICLES

机译：DNA功能化的Pd纳米粒子的制备

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Herein, Pd nanoparticles (NPs) with the average size of 10 ran were firstly obtained by using sodium citrate as the stabilizing ligand and ethanol as reducing agent In order to link DNA to Pd NPs surface through amide bond linkage or S-Pd bond, the different surface modifications (protected by TA (thioctic acid), MUDA (11-mercaptoundecanoic acid), HS-PEG (MW 2 000)) through the ligand exchange reaction were then done. And during the subsequent attachment of DNA, it was found that only Pd NPs modified with HS-PEG and HS-DNA simultaneously performed the good stability in the different buffer solution, which is very different from the protocol used in traditional Au NPs or QD surface modification. And this work set the good foundation for the application of Pd-DNA conjugate in further immunoassay and nucleic acid hybridization.

机译：在此，首先以柠檬酸钠为稳定配体，以乙醇为还原剂，制备平均粒径为10nm的Pd纳米颗粒。为了通过酰胺键或S-Pd键将DNA连接到Pd NPs表面，然后通过配体交换反应进行了不同的表面改性（用TA（硫辛酸），MUDA（11-巯基癸酸），HS-PEG（MW 2000）保护）。并且在随后的DNA连接过程中，发现只有用HS-PEG和HS-DNA修饰的Pd NP在不同的缓冲溶液中同时具有良好的稳定性，这与传统的Au NP或QD表面所使用的方案大不相同。修改。这项工作为在进一步的免疫测定和核酸杂交中应用Pd-DNA缀合物奠定了良好的基础。

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来源
《Progress on post-genome technologies and modern natural products》|2011年|123-125|共3页
会议地点 Chongqing(CN)
作者
Li Hongying; Wang Zhifei; He Nongyue;
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作者单位

School of Chemsitry and Chemical Engineering, Southeast University, Nanjing 211189, China;

School of Chemsitry and Chemical Engineering, Southeast University, Nanjing 211189, China;

State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
关键词
Palladium nanoparticles (Pd NPs); DNA; Stability;

机译：钯纳米粒子（Pd NPs）;脱氧核糖核酸;稳定性;

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PREPARATION OF DNA-FUNCTIONALIZED Pd NANOPARTICLES

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