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Coherent label-free imaging through turbidity: a holographic approach

机译：通过浊度实现无标签相干成像：全息方法

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摘要

A challenging issue has to be faced in microscopy whenever samples are immersed inside a dynamic turbid medium, as occluding objects provoke severe light scattering or unpredictable time-variable phase delays that scramble the object information. In these cases, the transmission matrix of the medium cannot be fully characterized. Here we show a simple technique, named Multi-Look Digital Holography (MLDH), able to fully recover the useful signal of biological specimens dipped inside a turbid liquid phase. Multiple hologram recordings are incoherently combined to synthesize the whole complex field diffused by the sample, which is revealed through turbidity providing quantitative phase-contrast information. Moreover, we show that the presence of a turbid medium can have a positive effect on a coherent imaging system, helping to reduce the effect of speckle artifacts. In other words, occluding biological elements, like e.g. Red Blood Cells (RBCs), can be thought as useful optical components providing denoising capabilities.
机译：每当将样品浸入动态混浊介质中时，显微技术都必须面对一个具有挑战性的问题，因为阻塞物体会引起严重的光散射或扰乱物体信息的不可预测的时变相位延迟。在这些情况下，无法完全表征介质的传输矩阵。在这里，我们展示了一种简单的技术，称为多用途数字全息（MLDH），它能够完全恢复浸入浑浊液相中的生物样本的有用信号。多个全息图记录不相干地组合在一起，以合成样品扩散的整个复杂场，这通过浊度显示出来，从而提供定量的相衬信息。此外，我们表明混浊介质的存在可以对相干成像系统产生积极影响，有助于减少斑点伪影的影响。换句话说，封闭生物元素，例如红细胞（RBC）可以被视为提供去噪功能的有用光学组件。

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  • 来源
    《Quantitative phase imaging II》|2016年|971807.1-971807.8|共8页
  • 会议地点 San Francisco CA(US)
  • 作者

    V. Bianco; M. Paturzo; V. Marchesano; L. Miccio; P. Memmolo; P. Ferraro;

  • 作者单位

    Institute of Applied Sciences and Intelligent Systems, Italian National Research Council (ISASI-CNR), Via Campi Flegrei, 34, Ⅰ-80078, Pozzuoli (NA), Italy;

    Institute of Applied Sciences and Intelligent Systems, Italian National Research Council (ISASI-CNR), Via Campi Flegrei, 34, Ⅰ-80078, Pozzuoli (NA), Italy;

    Institute of Applied Sciences and Intelligent Systems, Italian National Research Council (ISASI-CNR), Via Campi Flegrei, 34, Ⅰ-80078, Pozzuoli (NA), Italy;

    Institute of Applied Sciences and Intelligent Systems, Italian National Research Council (ISASI-CNR), Via Campi Flegrei, 34, Ⅰ-80078, Pozzuoli (NA), Italy;

    Institute of Applied Sciences and Intelligent Systems, Italian National Research Council (ISASI-CNR), Via Campi Flegrei, 34, Ⅰ-80078, Pozzuoli (NA), Italy;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    (090.1995) Digital holography; (290.7050) Scattering: turbid media; (110.0113); Imaging through turbid media; (030.4280) Noise in imaging systems; (030.6140) Speckle; (110.4190) Multiple imaging;

    机译：（090.1995）数字全息术； （290.7050）散射：介质混浊； （110.0113）；通过混浊介质成像； （030.4280）成像系统中的噪声； （030.6140）斑点; （110.4190）多重成像;

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