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Micro patterned surfaces -an effective tool for long term Digital Holographic Microscopy cell imaging

机译:微图案化表面-长期数字全息显微细胞成像的有效工具

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The major problem of Digital Holographic Microscopy (DHM) long term live cell imaging is that over time most of the tracked cells move out of the image area and other ones move in. Therefore, most of the cells are lost for the evaluation of individual cellular processes. Here, we present an effective solution for this crucial problem of long-term microscopic live cell analysis. We have generated functionalized slides containing areas of 250 μm per 200 μm. These micropatterned biointerfaces consist of passivating polyaclrylamide brushes (PAAm). Inner areas are backfilled with octadecanthiol (ODT), which allows cell attachment. The fouling properties of these surfaces are highly controllable and therefore the defined areas designed for the size our microscopic image areas were effective in keeping all cells inside the rectangles over the selected imaging period.
机译:数字全息显微术(DHM)长期活细胞成像的主要问题是,随着时间的流逝,大多数被跟踪的细胞会移出图像区域,而其他的则会移入图像区域。因此,大部分细胞会丢失,无法评估单个细胞流程。在这里,我们为长期的微观活细胞分析这一关键问题提出了一种有效的解决方案。我们已经生成了每200μm包含250μm面积的功能化载玻片。这些微图案化的生物界面由钝化聚丙烯酰胺刷(PAAm)组成。内区用十八烷硫醇(ODT)填充,从而允许细胞附着。这些表面的结垢特性是高度可控的,因此,为我们的显微图像区域设计的定义区域有效地将所有单元格保持在选定的成像周期内。

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